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Semi-quantitative visualized ELISA analysis method

An enzyme-linked immunoassay and semi-quantitative technology, applied in the field of semi-quantitative visual enzyme-linked immunoassay, can solve the problems of development and application lag, lack of commercialized instruments, reports, etc., and achieve low detection limit, high reproducibility, The effect of easy access to raw materials

Active Publication Date: 2016-01-06
金瑞博(厦门)生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0004] Although SPR detection technology has very good application prospects in the field of ELISA, compared with traditional ELISA, the development and application of surface plasmon resonance-based enzyme-linked immunosorbent assay technology is relatively lagging behind, so far there is no related commercial product. chemical instrument report

Method used

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Embodiment 1

[0025] The following examples combine Attached picture To illustrate the operation process of applying the microfluidic chip prepared by the present invention to carry out actual sample analysis:

[0026] (1) Before the synthesis, soak all the required glassware with aqua regia, then rinse with a large amount of water, and finally rinse with ultrapure water;

[0027] (2) Synthesis of gold nanoparticles with a particle size of 15±5nm: synthesized by sodium citrate reduction method, 100mL, 2.5×10 -4 M chloroauric acid was placed in a 120°C oil bath and refluxed for 30min; then 10mL of 1wt% sodium citrate solution was added to it, reacted for 20min under stirring, then turned off the heating, and continued to stir and reflux to room temperature.

[0028] (3) Synthesis of nano-gold with a particle size of 45±15nm: synthesized by the gold seed growth method, first prepare 60mL of growth solution, which contains 2.5×10 chloroauric acid -4 M, cetyltrimethylammonium bromide (CTAB) ...

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Abstract

The invention discloses a novel semi-quantitative visualized ELISA analysis method. The analysis method comprises the following steps: (1) a preparation of chromogenic nano-gold; (2) an ELISA adsorption and color development reaction. The invention has the advantages of easily available raw materials and low cost, complex detection equipments are not needed, and detection result can be determined by naked eyes. The method has the advantages of simple operation method, good reappearance and good stability. The method can be directly applied to semi-quantitative rapid detection of various clinic marker proteins, and the widely applied to life science and clinical medicine detection and other fields.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a semi-quantitative visualized enzyme-linked immunoassay method. Background technique [0002] Enzyme Linked ImmunoSorbent Assay (EnzymeLinkedImmunoSorbentAssay), referred to as ELISA, uses the specific reaction of antigen and antibody to connect the analyte to the enzyme, and then produces a color reaction through the enzyme and the substrate, and performs qualitative or quantitative analysis of the tested substance. Detection method. Because of its fast, sensitive, simple, easy standardization and other advantages, it has been developed rapidly and widely used. It is an experimental technique with high specificity and high sensitivity, which is based on immunological reactions and combines the specific reactions of antibodies and antigens with the efficient catalytic action of enzymes on substrates. [0003] The detection result of the enzyme-linked immunosorbent assay lie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/78
CPCG01N21/78G01N33/53
Inventor 郭隆华许梢华马小明林振宇邱彬陈国南
Owner 金瑞博(厦门)生物科技有限公司
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