Semi-quantitative visualized ELISA analysis method
An enzyme-linked immunoassay and semi-quantitative technology, applied in the field of semi-quantitative visual enzyme-linked immunoassay, can solve the problems of development and application lag, lack of commercialized instruments, reports, etc., and achieve low detection limit, high reproducibility, The effect of easy access to raw materials
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[0025] The following examples combine Attached picture To illustrate the operation process of applying the microfluidic chip prepared by the present invention to carry out actual sample analysis:
[0026] (1) Before the synthesis, soak all the required glassware with aqua regia, then rinse with a large amount of water, and finally rinse with ultrapure water;
[0027] (2) Synthesis of gold nanoparticles with a particle size of 15±5nm: synthesized by sodium citrate reduction method, 100mL, 2.5×10 -4 M chloroauric acid was placed in a 120°C oil bath and refluxed for 30min; then 10mL of 1wt% sodium citrate solution was added to it, reacted for 20min under stirring, then turned off the heating, and continued to stir and reflux to room temperature.
[0028] (3) Synthesis of nano-gold with a particle size of 45±15nm: synthesized by the gold seed growth method, first prepare 60mL of growth solution, which contains 2.5×10 chloroauric acid -4 M, cetyltrimethylammonium bromide (CTAB) ...
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