Bioactive peptide and application thereof

A bioactive peptide and antioxidant activity technology, applied in the field of active peptide screening, can solve the problems of low processing utilization, waste of protein resources, and backward mussel processing technology.

Inactive Publication Date: 2016-01-27
QINGDAO TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The output of mussels in my country ranks first in the world, and 90% of mussel products are used for export of fresh products. At present, due to the relatively backward processing technology of mussels, the processing utilization rate of mussels is low, the added value of products is low, and its protein resources are seriously wasted.

Method used

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  • Bioactive peptide and application thereof
  • Bioactive peptide and application thereof
  • Bioactive peptide and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0015] The screening of embodiment 1 active peptide

[0016] 1) Preparation of mussel protein hydrolyzate

[0017] Wash the fresh mussels to remove impurities such as silt, then soak and clean them with distilled water, remove the shells and shreds, add 2 times the amount (the weight of the mussel meat after treatment) to homogenate, freeze-dry the homogenate, Add 95% ethanol with a mass ratio of 1:5, degrease at 60°C for 4 hours, and repeat the degreasing operation 4 times. The degreased residue was vacuum-dried at 40° C., crushed, and passed through a 100-sieve to obtain mussel crude protein, whose protein content was determined to be 63.24%. Take mussel crude protein and add 3 times the amount of water, homogenize for 5 minutes, preheat to 50°C, add 5% animal protein hydrolysis special enzyme (Nanning Donghenghuadao Biotechnology Co., Ltd., calculated by protein content), stir the enzyme Decompose for 4 hours, heat at 100°C for 10 minutes to inactivate the enzyme, centrif...

Embodiment 2

[0027] The detection of embodiment 2 active peptide antioxidant activity

[0028] 1. Determination of Antioxidant Activity

[0029] Preparation of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) solution: Accurately weigh 4mg DPPH, dissolve it with methanol and set the volume in a 100ml volumetric flask, the concentration of DPPH is 0.004%, and store in the dark (0 ~4°C). Take the prepared active peptide sample solution 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml (the insufficient volume is filled to 1.0ml with the corresponding blank solvent) and 4ml 0.004% DPPH solution into the same test tube, shake well, Place it in the dark for 30min, measure its absorbance A at 517nm with the corresponding blank solvent as a blank, calculate its clearance rate: clearance rate I%=[(A 0 -A 1 ) / A 0 ]×100%

[0030] In the formula: A 0 - Absorbance of 1mL blank solvent plus 4.0ml DPPH solution;

[0031] A 1 - The absorbance of the solution to be tested plus 4.0ml DPPH solution.

[0032] IC 50 I...

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Abstract

The invention provides a bioactive peptide and application thereof, and belongs to the technical field of marine organisms. According to the bioactive peptide and the application thereof, seafood mussels which are common in coastal areas in Shandong province serve as the raw materials, a biological enzymolysis technology, a chromatographic separation technology and an amino acid sequence analysis technology are adopted to prepare a new short peptide containing six amino acid through separation and purification, the amino acid sequence is identified as Phe-Thr-Tyr-Val-Pro-Gly, and meanwhile in vitro activity shows that the bioactive peptide has strong antioxidant activity. The invention aims at providing a theoretical basis for development and utilization of mussel resources.

Description

technical field [0001] The invention belongs to the technical field of active peptide screening, and in particular relates to a bioactive peptide and its application. Background technique [0002] Mussel (Mytilusedulis), commonly known as "sea red" and "mussel", is a common seafood with high protein, low fat, rich in various nutrients and widely distributed. The output of mussels in my country ranks first in the world, and 90% of mussel products are used for fresh export. At present, due to the relatively backward processing technology of mussels, the processing utilization rate of mussels is low, the added value of products is low, and its protein resources are seriously wasted. The invention aims at discovering bioactive peptides from mussel protein hydrolyzate by using modern food processing technology and chromatographic technology, so as to provide a theoretical basis for its subsequent development and utilization of resources. Contents of the invention [0003] Aimi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/16A23L33/18A23K20/147A61K38/08A61P39/06
Inventor 耿传信杨海刘雪丽
Owner QINGDAO TUMOR HOSPITAL
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