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A kind of SNP marker related to the prevention of colorectal cancer by non-steroidal anti-inflammatory drugs and its application

A colorectal cancer, non-steroidal technology, applied in the field of SNP markers, can solve the problems of colorectal cancer preventive drug guidance and auxiliary diagnosis without SNP application

Active Publication Date: 2018-12-07
NANJING YIKE POPULATION HEALTH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of NSAID-related SNPs in the guidance of preventive medication and auxiliary diagnosis of colorectal cancer.

Method used

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  • A kind of SNP marker related to the prevention of colorectal cancer by non-steroidal anti-inflammatory drugs and its application
  • A kind of SNP marker related to the prevention of colorectal cancer by non-steroidal anti-inflammatory drugs and its application
  • A kind of SNP marker related to the prevention of colorectal cancer by non-steroidal anti-inflammatory drugs and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 Sample collection and sample data collation

[0084] The inventors selected 1202 samples that met the following criteria to perform a single SNP Sequenom MassARRAY genotyping test sample:

[0085] 1. 568 patients with colorectal cancer diagnosed by pathology;

[0086] 2. 634 healthy controls matched with the age and gender of the case;

[0087] The demographic data and clinical data of these samples were collected systematically, and the clinical course records were used to extract whether the individuals were regularly taking non-steroidal anti-inflammatory drugs. 362 patients took non-steroidal anti-inflammatory drugs regularly. , 206 patients did not take non-steroidal anti-inflammatory drugs. The scope of drugs includes acetylsalicylate (such as aspirin), non-salicylate and non-acetylsalicylate drugs (such as fenbid, ibuprofen, nimesulide, voltarin, etc.).

Embodiment 2

[0088] Example 2 Type scanning of SNP markers in peripheral blood DNA

[0089] Among the above-mentioned 568 eligible patients with colorectal cancer (including 362 in the non-steroidal anti-inflammatory drug group and 206 in the non-steroidal anti-inflammatory group) and 634 normal controls, the case-control age and gender matched. These two groups of people were tested by Sequenom MassARRAY to obtain relevant results. The specific steps are:

[0090] 1. Add hemolysis reagent (i.e. lysate, 40 parts preparation method is as follows: sucrose 219.72g, magnesium chloride 2.02g and Triton X-100 (amresco 0694) 20ml) to the peripheral blood stored in the 2ml cryotube. Dilute the TrisHcl solution to 2000ml, the same below), mix it upside down and transfer it in completely.

[0091] 2. Removal of red blood cells: make up the 5ml centrifuge tube to 4ml with hemolysis reagent, mix upside down, centrifuge at 4000rpm for 10 minutes, discard the supernatant. Add 4ml of hemolysis reagent to the...

Embodiment 3

[0110] Example 3 Use of risk score method to further analyze the relationship between SNP and colorectal cancer

[0111] Based on the above results, the present inventors analyzed the stratification factors of whether or not to take non-steroidal anti-inflammatory drugs in the two groups of samples (ie, "the group taking non-steroidal anti-inflammatory drugs for colorectal cancer" and "not taking non-steroidal anti-inflammatory drugs". Colorectal cancer group"), the genotype distribution frequency is compared, and 15 positively associated SNPs are finally selected, and the single SNP regression coefficient is used as the weight to further obtain the risk score, and draw the ROC to evaluate the sensitivity and sensitivity of diagnosis. Specificity, and then analyze whether these SNPs are suitable for taking non-steroidal anti-inflammatory drugs to prevent the onset of colorectal cancer for diagnosis. The combined analysis of 15 SNP markers found that these 15 SNPs separate colorec...

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Abstract

The invention belongs to the field of genetic engineering and tumor medicine, and discloses an SNP (single nucleotide polymorphism) marker relevant to prevention of colorectal cancer by the aid of non-steroidal anti-inflammatory medicines and application of the SNP marker. The SNP marker is a composition of rs12217975, rs1927466, rs7586006, rs17868336, rs1500482, rs16973225, rs10505806, rs6717546, rs2965667, rs73172231, rs16825767, rs1902023, rs2745559, rs73172243 and rs2920421. The SNP marker and the application have the advantages that the SNP marker can be used for guiding prevention and treatment clinical medication of the non-steroidal anti-inflammatory medicines for colorectal patients, accordingly, medicine abuse can be reduced, and excessive medical treatment can be prevented.

Description

Invention field [0001] The invention belongs to the fields of genetic engineering and oncology medicine, and relates to SNP markers related to the prevention of colorectal cancer by non-steroidal anti-inflammatory drugs and applications thereof. Background technique [0002] Colorectal cancer is one of the most common gastrointestinal tumors in the world. It is the third most common tumor in men and the second in women, and it ranks second in cancer deaths. According to estimates by the International Agency for Research on Cancer of the World Health Organization, there were about 1.2 million new cases of colorectal cancer worldwide in 2008, and the mortality rate accounted for about 8% of all malignant tumors. In my country, with the development of society and economy and the continuous changes in residents’ lifestyles and dietary habits, the incidence and mortality of colorectal cancer have shown an upward trend year by year, ranking third in the incidence of malignant tumors an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/106C12Q2600/156
Inventor 王一雄江玥戴俊程李绵丁舒丹
Owner NANJING YIKE POPULATION HEALTH RES INST CO LTD
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