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Single-channel optical super-resolution imaging instrument

A super-resolution imaging, single-channel technology, applied in the field of super-resolution imaging, can solve problems such as multi-channel excitation and complexity, and achieve the effects of easy and precise control, simplified complexity, enhanced usability and scalability

Active Publication Date: 2016-02-03
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a single-channel optical super-resolution imaging instrument, which is used to solve the problem of the complexity of multi-channel excitation of super-resolution imaging (STORM) technology in the prior art

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Examples

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Embodiment 1

[0051] Such as Figure 1 ~ Figure 2 As shown, this embodiment provides a single-channel optical super-resolution imaging instrument, the single-channel optical super-resolution imaging instrument at least includes an excitation light source module 1 and an imaging, scanning and detection module 2;

[0052] The laser 11, the acousto-optic modulator 12, and the fiber coupler 13 included in the excitation light source module 1; the laser 11, the acousto-optic modulator 12, and the fiber coupler 13 are arranged in sequence; the laser 11, the acousto-optic modulator 12. The optical axes of the frequency scanning and selection device and the fiber coupler 13 are on the same straight line;

[0053] The imaging, scanning and detection module 2 includes two lenses 21, a polarizing beam splitter 22, a microscope objective 23, a sample stage 24 and an imaging device 25; the two lenses 21, a polarizing beam splitter 22 and a microscope objective The optical axes of 23 are on the same str...

Embodiment 2

[0060] Such as Figure 3 ~ Figure 5 As shown, this embodiment provides a single-channel optical super-resolution imaging instrument, the single-channel optical super-resolution imaging instrument at least includes an excitation light source module 1 and an imaging, scanning and detection module 2;

[0061] The laser 11, the acousto-optic modulator 12, and the fiber coupler 13 included in the excitation light source module 1; the laser 11, the acousto-optic modulator 12, and the fiber coupler 13 are arranged in sequence; the laser 11, the acousto-optic modulator 12. The optical axes of the frequency scanning and selection device and the fiber coupler 13 are on the same straight line;

[0062] The imaging, scanning and detection module 2 includes two lenses 21, a polarizing beam splitter 22, a microscope objective 23, a sample stage 24 and an imaging device 25; the two lenses 21, a polarizing beam splitter 22 and a microscope objective The optical axes of 23 are on the same str...

Embodiment 3

[0074] Such as Figure 6 ~ Figure 8 As shown, this embodiment provides a single-channel optical super-resolution imaging instrument, the single-channel optical super-resolution imaging instrument at least includes an excitation light source module 1 and an imaging, scanning and detection module 2;

[0075] The laser 11, the acousto-optic modulator 12, and the fiber coupler 13 included in the excitation light source module 1; the laser 11, the acousto-optic modulator 12, and the fiber coupler 13 are arranged in sequence; the laser 11, the acousto-optic modulator 12. The optical axes of the frequency scanning and selection device and the fiber coupler 13 are on the same straight line;

[0076] The imaging, scanning and detection module 2 includes two lenses 21, a polarizing beam splitter 22, a microscope objective 23, a sample stage 24 and an imaging device 25; the two lenses 21, a polarizing beam splitter 22 and a microscope objective The optical axes of 23 are on the same str...

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Abstract

The invention provides a single-channel optical super-resolution imaging instrument which at least comprises an excitation light source module and an imaging, scanning and detecting module, wherein the excitation light source module comprises an optical laser, an acousto-optic modulator and an optical fiber coupler; the optical laser, the acousto-optic modulator and the optical fiber coupler are arranged in sequence; the optical axes of the optical laser, the acousto-optic modulator, a frequency scanning and selecting device and the optical fiber coupler are positioned on the same straight line; the imaging, scanning and detecting module comprises two lenses, a polarization beam splitter, a microscope objective, a sample stage and an imaging device; the optical axes of the lenses, the polarization beam splitter and the microscope objective are positioned on the same straight line and the optical axes of the imaging device and the polarization beam splitter are positioned on the same straight line. According to the invention, a single-channel frequency scanning, resonance excitation and polarization filtering combined method is adopted, so that the single-channel excitation super-resolution imaging of samples is realized. The single-channel optical super-resolution imaging instrument provided by the invention is simple in structure, obvious in effect and applied to the technical field of experiential and industrial super-resolution imaging.

Description

technical field [0001] The invention relates to the technical field of super-resolution imaging, in particular to a single-channel optical super-resolution imaging instrument. Background technique [0002] Today's nanotechnology is increasingly becoming the frontier of natural science and technology development, such as the construction of new electronics and new photonics at the nanometer level, the synthesis of new surface catalysts at the nanometer level, and the wide use of new nanoparticles for biological (fluorescence) labeling Wait. The development of nanotechnology is inseparable from nanomicroscopic imaging technology, which has become the standard configuration of many laboratories represented by scanning electron microscope and atomic force microscope. However, optical detection technology has a strong advantage in studying microscopic internal states such as electronic structures, phonon states, and plasmons at the nanoscale. However, limited by the optical diff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王中阳赵建伟沈灏
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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