A kind of preparation method of deer blood active component

An active component, the technology of deer blood, applied in the field of preparation of deer blood active components, can solve the problems of restricting the consumption of deer blood, and achieve good application prospects, eliminate allergens, and wide application prospects

Active Publication Date: 2018-08-31
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In ancient my country, as early as the Song Dynasty, the nobles of the palace raised deer blood for raw drinks. However, this way of eating limited the number of people who can eat deer blood. It is urgent to develop deer blood health products that are convenient to eat and have clear effects, so that deer blood, a royal nourishment Products into the families of ordinary people

Method used

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  • A kind of preparation method of deer blood active component
  • A kind of preparation method of deer blood active component
  • A kind of preparation method of deer blood active component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preparation of Active Components of Deer Blood

[0020] Take 10 liters and add 20 grams of anticoagulant EDTA-K.2H 2 O fresh red deer deer blood, add 10 liters of deionized water, mix well, heat to 80°C, stir and keep warm for 10 minutes, cool down to 40°C, adjust pH to 2.0 with 1M HCI, add 0.2 g of pepsin, and stir for 24 hours; After enzymolysis, heat up to 80°C, stir and keep warm for 10 minutes, then cool down to 40°C, add 1 gram of active dry yeast for brewing, and ferment for 24 hours; after fermentation, heat up to 100°C, keep warm for 10 minutes; after the reaction, freeze-dry to obtain Powdered deer blood active ingredient.

[0021] 2. Antioxidant capacity of active components of deer blood

[0022] 2.1 Determination of antioxidant activity

[0023] 2.1.1 Determination of iron reducing ability

[0024] Fe 3+ -Tripyridine-triazine (TPTZ) can be reduced to Fe by reducing species in the sample 2+ It is blue and has a maximum absorbance value at 593nm. Acc...

Embodiment 2

[0055] Take 100 liters of fresh sika deer blood with 15000U anticoagulant heparin, add 100 liters of deionized water, mix well, heat to 100°C, stir and keep warm for 10 minutes; cool down to 50°C, adjust pH to 8.0 with 1M NaOH, add 500g Alkaline protease, stirred for 5 hours; after the end of the enzymolysis, the temperature was raised to 80°C, stirred and kept for 10 minutes, then the temperature was lowered to 30°C, and 500 grams of brewing active dry yeast was added, and fermented for 12 hours; after the fermentation, the temperature was raised to 100°C, heat preservation for 10 minutes; after the reaction, freeze-dry to obtain the active component of deer blood in powder form.

[0056] The activity detection result of the active component of deer blood is the same as that of Example 1.

Embodiment 3

[0058] Take 10 liters of fresh sika deer antler blood with 20 grams of anticoagulant sodium oxalate, add 10 liters of deionized water, mix well, heat to 60°C, stir and keep warm for 60 minutes, cool down to 55°C, adjust the pH to 8.0 with 1M NaOH, add 2 grams of bromelain and papain mixture (the mass ratio of the two is 1:1), stirring for 10 hours for enzymolysis; after the end of enzymolysis, heat up to 80°C and stir for 10 minutes, then cool down to 45°C, add 20 grams of wine-making activity Dried yeast, fermented for 8 hours; after the fermentation was completed, the temperature was raised to 100° C., and kept for 10 minutes; after the reaction was completed, the powdered deer blood active component was obtained by freeze-drying.

[0059] The activity detection result of the active component of deer blood is the same as that of Example 1.

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Abstract

The invention relates to a preparation method of active components of deer blood. The invention uses fresh deer blood as a raw material, and obtains the active component of deer blood after virus inactivation, protease hydrolysis once, brewing yeast twice fermentation and drying. The active components of deer blood prepared by the technology of the present invention have the following characteristics: (1) Enzymolysis degrades large molecular weight proteins into small molecules, which not only avoids adverse reactions such as allergies caused by deer blood, but also exposes the active site of the peptide to have ACE inhibitory activity, DPP‑IV inhibitory activity, antibacterial activity and other functions; (2) The active components of deer blood after the first enzymolysis and second fermentation eliminate the original fishy smell of blood products, and the taste is easier to be consumed accept. The active components of deer blood can be used in pharmaceuticals, functional foods, food additives and other fields, and have broad application prospects.

Description

technical field [0001] The invention relates to the preparation technology of active components of deer blood. Background technique [0002] Deer blood has been a precious tonic since ancient times. Chinese herbal books such as "Compendium of Materia Medica" and "New Edition of Materia Medica" have records of the medicinal value of deer blood. The use of deer blood for fitness was first seen in the "Qingbo Magazine" in the Song Dynasty. The "Compendium of Materia Medica" written by Li Shizhen in the Ming Dynasty recorded the medical effects of deer blood in detail: "mainly impotence, tonic, lumbago, falls, rabies, and Take alcohol to control lung impotence, hematemesis, and collapse, and those who are suffering from qi pain and want to be in danger drink it to heal immediately. It can greatly tonify deficiency and damage, benefit essence and blood, relieve acne poison, and drug poison. In ancient my country, as early as the Song Dynasty, the royal palace and nobles used it Br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/14A23L33/00A61P39/06A61P37/04A61P35/00A61P9/12
Inventor 邹汉法靳艳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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