A gel-free sieving medium for capillary electrophoresis and its preparation method

A technology of capillary electrophoresis and sieving, which is applied in biochemical equipment and methods, DNA preparation, microbial measurement/inspection, etc. It can solve the problems of poor stability and low resolution, achieve high hydrophilicity, reduce adsorption, Ease of injection and replacement of media
CN105367693BActive Publication Date: 2018-02-02THE FIRST RES INST OF MIN OF PUBLIC SECURITY +1
9 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST RES INST OF MIN OF PUBLIC SECURITY
Publication Date
2018-02-02

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the technical field of capillary electrophoreses, in particular to a non-gel sieving medium for capillary electrophoresis and a preparation method thereof. The non-gel sieving medium for capillary electrophoresis comprises a polymer monomer which is acrylamide, solvent which is deionized water, a chain transfer agent which is isopropanol, an initiator which is ammonium persulfate and a catalyst which is an N,N,N,N-tetramethylethylenediamine mixture. The preparation method of the non-gel sieving medium for capillary electrophoresis comprises the steps that the polymer monomer, the solvent, the chain transfer agent, the initiator and the catalyst are mixed, and after dialysis and freeze drying are conducted, the mixture is swelled in a sol buffer solution, wherein the sieving medium is polyacrylamide. The prepared sieving medium has good sieving performance, dissolving capacity and high hydrophilicity, the adsorptive action between DNA and the capillary wall is effectively reduced, the high molecular weight and low viscosity are achieved, medium injection and replacement are convenient, automation of DNA analysis and separation is facilitated, and the requirements of legal medical experts on STR analysis and detection can be met.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of sieving media for capillary electrophoresis, in particular to a gel-free sieving medium for capillary electrophoresis and a preparation method thereof. Background technique

[0002] In the prior art, capillary electrophoresis (capillary electrophoresis, CE), also called high-performance capillary electrophoresis (HPCE), is one of the fastest-growing analytical methods in recent years. In 1981, Jorgenson and Lukacs first proposed to separate by applying high voltage in a capillary column with an inner diameter of 75 μm, and created modern capillary electrophoresis. In 1984, Terabe et al established micellar capillary electrokinetic chromatography. In 1987, Hjerten established capillary isoelectric focusing, and Cohen and Karger proposed capillary gel electrophoresis. From 1988 to 1989, the first batch of commercial capillary electrophoresis instruments appeared. In just a few years, because capillary el...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More