A method for outdoor large-scale photoautotrophic two-stage cultivation of oleaginous green algae
A two-stage, photoautotrophic technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of high cost and no related reports, so as to increase oil content and increase oil production , The effect of reducing the cost of cultivation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] A method for outdoor large-scale photoautotrophic two-stage cultivation of oleaginous green algae, comprising the following steps:
[0025] 1. Stock preparation
[0026] 1). Gradually expand and cultivate the algae species, and the algae species in the test tube are expanded to 500mL.
[0027] 400mL of liquid medium was divided into 500mL triangular flasks, sealed with tin foil and then sterilized. Chlorella sp. (preserved in the Freshwater Algae Species Bank of Chinese Academy of Sciences, No. FACHB-1748) was inoculated into 500mL triangular flasks, and the inoculum OD was 0.05, air is introduced, at the beginning, the air flow is 0.3L / min, and the light intensity does not exceed 400μmol m -2 the s -1 , until the color in the flask turns green and the OD is 0.5, increase the ventilation rate to 0.8 L / min. The light intensity can also be increased slowly, and the light intensity is controlled at less than 1000μmol m -2 the s -1 . See Table 1 for liquid culture med...
Embodiment 2
[0033] A method for outdoor large-scale photoautotrophic two-stage cultivation of oleaginous green algae, comprising the following steps:
[0034] The culture medium used in this embodiment is shown in Table 1. This embodiment takes the columnar photobioreactor erected in an outdoor solar panel greenhouse as an example for illustration:
[0035] 1. Inoculate the algae cultivated to the logarithmic phase into a 140L columnar photobioreactor (the 140L reactor is composed of two 70L columnar photobioreactors connected through the channel at the bottom, and the specification of the 70L reactor is: height 1.83 m, with a diameter of 0.22m, Dalian Huixin Titanium Equipment Development Co., Ltd.). The liquid volume of the columnar photobioreactor is 120L, and the inoculation OD is 0.3. A 3L13XD Roots blower was used to add carbon dioxide to the columnar photobioreactor for aeration and bubbling through the inflatable pipeline. The ventilation rate was 6L / min, and the temperature in t...
Embodiment 3
[0053] Preparation of algae species for large-scale cultivation in racetrack ponds:
[0054] The steps of preparing the original seed and expanding the cultivation to a 5L Erlenmeyer flask are the same as in Example 1.
[0055] The 18L transparent barrel is divided into 15L culture solution, and the logarithmic algae in the 5L triangular flask are inoculated into the 18L transparent barrel. The vent pipe is connected to the sterilized sand core, and it is directly passed into the bottom of the barrel for ventilation (the ventilation rate is 1L / min) culture, the aeration period is 6:00-20:00, and the rest of the time is static culture, control the light intensity ≤ 1400μmol m -2 the s -1 , the algal species cultivated to the logarithmic phase are used in the following examples.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More