Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting effective components of girald daphne bark

A technology of active ingredient and determination method, applied in the field of active ingredient determination of Zu Shima, can solve the problem of fingerprints without any reports and the like

Inactive Publication Date: 2016-03-23
QINHUANGDAO SHANHAIGUAN PHARMA CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In recent years, there are many documents about the quality control of Zushima, but there is no report on the study of fingerprints. After research, the present invention finds a method for content determination that is especially suitable for Zushima preparations, and establishes a more comprehensive and more comprehensive detection method. Rapid Quality Evaluation Method of Zushima

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting effective components of girald daphne bark
  • Method for detecting effective components of girald daphne bark
  • Method for detecting effective components of girald daphne bark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0209] Zushima tablet of the present invention, preparation method is as follows:

[0210] Take 1100g Zushima, add water and decoct three times, the first time for 2 hours, the second time, and the third time for 1 hour, combine the decoction, filter, and concentrate the filtrate to a clear paste with a relative density of 1.22-1.26 (50°C). Add ethanol to make the alcohol content reach 75%, let it stand for precipitation, take the supernatant, recover the ethanol and concentrate to an appropriate amount, add 10g of calcium carbonate, appropriate amount of starch, and dextrin, mix well, make granules, dry, and press into 1000 Tablets, film-coated or sugar-coated, ready to serve.

Embodiment 2

[0212] The active ingredient assay method of the zushima preparation of the present invention comprises the following steps:

[0213] 1) Preparation of the reference substance solution Take an appropriate amount of the reference substance of ancestralin and 7-hydroxycoumarin, accurately weigh it, add 85% methanol to make each 1ml contain 0.1 mg of ancestralin and 20 μg of 7-hydroxycoumarin The mixed solution, that is. 2) Preparation of the test solution Take 20 tablets of this product, remove the coating, accurately weigh, grind finely, take about 0.6g,

[0214] Accurately weighed, put in a stoppered Erlenmeyer flask, accurately add 50ml of 85% methanol, seal the plug, weigh, ultrasonically treat (power 500W, frequency 40kHz) for 30 minutes, let cool, weigh again, and use 85% methanol Make up for the lost weight, shake well, filter, and take the filtrate to get it.

[0215] 3) Determination method Precisely draw 10 μl of each of the reference substance solution and the test ...

Embodiment 3

[0221] The identification method of ancestral hemp preparation, described method, comprises the following steps:

[0222] 1) Take 2 Zushima tablets, remove the coating, grind finely, put them in a stoppered Erlenmeyer flask, add 25ml of methanol, sonicate for 20 minutes, filter, and concentrate the filtrate to about 1ml as the test solution.

[0223] 2) Take 1 g of Zushima reference medicinal material, and prepare the reference medicinal material solution in the same way.

[0224] 3) Then take the zushimarin reference substance, add methanol to make a solution containing 1mg per 1ml, and use it as the reference substance solution.

[0225] 4) According to the test of thin-layer chromatography (Appendix VIB of "Chinese Pharmacopoeia" 2010 Edition), draw 2-5 μl of each of the above-mentioned three solutions, and 5 μl of the control extract solution, respectively spot on the same silica gel GF254 thin-layer plate, and use three Chloromethane-acetone-glacial acetic acid (7:1:0.5)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting effective components of girald daphne bark. The method includes the steps of firstly, preparing a reference substance solution, wherein a mixed solution containing daphnetin and umbelliferone is prepared from daphnetin and umbelliferone reference substances; secondly, preparing a test article solution, wherein girald daphne bark tablets are taken to be fine ground, solvent is added, ultrasonic treatment is conducted, and then filtering is conducted to obtain subsequent filtrate; thirdly, conducting detecting, wherein the reference substance solution and the test article solution are taken to be injected into a liquid chromatograph, a chromatogram is obtained, and the contents of the corresponding components in a test article are calculated according to the chromatogram.

Description

Technical field: [0001] The present invention relates to a detection method of medicines, in particular to a detection method of effective components of Zushima Background technique: [0002] Zushima is the root bark and stem bark of Daphne genus Daphne in the family Daphneaceae. It is also known as Zushima, Huang Ruixiang, Dajiujia, Jindai, and Huanggoupi. This product is an unused Chinese herbal medicine, but it is widely used after being made into a Chinese patent medicine. It is widely used in folks to treat pain, bruises, rheumatoid arthritis and bronchitis. [0003] Existing Zushima preparations include Zushima Tablets. Properties: This product is a film-coated tablet. After removing the film coating, the heart of the tablet is brown; the taste is slightly bitter. [0004] Functions and indications: expelling wind and dampness, promoting blood circulation and relieving pain. It is used for rheumatism blockage and arthralgia caused by blood stasis blocking collaterals...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/90
CPCG01N30/02G01N30/34G01N30/90G01N2030/027
Inventor 张旭张仲毅赵相友韩龙唐飞方向伟
Owner QINHUANGDAO SHANHAIGUAN PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com