Method for measuring glycosylated hemoglobin by isotope dilution mass spectrometry

A technology of glycosylated hemoglobin and isotope dilution, applied in the field of determination of glycosylated hemoglobin, can solve the problems of insufficient standardization of detection methods, not recommended for diabetes, and high maintenance and maintenance costs
CN105445409AActive Publication Date: 2016-03-30BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
Publication Date
2016-03-30

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Abstract

The present invention discloses a method for measuring glycosylated hemoglobin by isotope dilution liquid chromatography-tandem mass spectrometry. The method comprises the following steps: 1) a mole <15>N-HbA1c-<13>C and b mole <15>N-HbA0-<13>C both are added into a standard substance solution and a to-be-tested sample, the standard substance solution and the to-be-tested sample pass by a solid phase extraction column to obtain a to-be-loaded standard substance and a to-be-loaded to-be-tested sample, <15>N-HbA1c-<13>C is a hexapeptide chain at the N terminal of an isotope 15N and 13C-labeled glycosylated hemoglobin beta chain, the <15>N-HbA0-<13>C is a hexapeptide chain at the N terminal of an isotope 15N and 13C-labeled non-glycosylated hemoglobin beta chain, and a and b both are greater than 0; 2) the to-be-loaded standard substance and the to-be-loaded to-be-tested sample are respectively detected by a liquid chromatography-tandem mass spectrometer; and 3) the content of the glycated hemoglobin in the to-be-tested sample can be obtained from the testing results of the step 2). The method is simple in operation, is more accurate and precise than a mass spectrometric method recommended by IFCC, has good specificity and sensitivity, and is expected to become a decisive reference method for the determination of the glycated hemoglobin.
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Description

technical field

[0001] The invention relates to a method for measuring glycated hemoglobin by isotope dilution mass spectrometry, in particular to a method for accurately measuring the content of glycated hemoglobin in a sample to be tested by using isotope dilution liquid chromatography tandem mass spectrometry. Background technique

[0002] Glycosylated hemoglobin (abbreviated as hemoglobinA1c, HbA 1c , A1C or HbA1c) is the product of the combination of hemoglobin and glucose in blood red blood cells, which can reflect the average blood sugar level in the patient's body 6-8 weeks before the measurement. The proportion of hemoglobin being glycosylated is directly proportional to the level of blood glucose concentration in the body over a period of time. Correspondingly, non-glycosylated hemoglobin is represented by HbA0. Currently, the international definition of HbA 1c The chemical composition is that glucose is stably linked to the N-terminal valine (Val) residue of th...

Claims

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