Method for preparing tussah silk fibroin protein antibody using characteristic decapeptide

Abstract

The present invention discloses a method for using a characteristic decapeptide for preparing a tussah fibroin protein antibody, a synthetic sequence is a ''AAAAAAAAAA'' polypeptide, the polypeptide is coupled with keyhole limpet hemocyanin to obtain a full antigen; the full antigen is diluted with normal saline, the diluted full antigen is mixed evenly with Quick Antibody-Rabbit 5 w adjuvant, primary immunization is performed on a rabbit, then booster immunization is performed on the rabbit, reagents used for the booster immunization and the primary immunization are same; when titer of the antibody in a blood sample of the rabbit reaches 1: 10,000, blood of the immunized rabbit is collected, blood clots are fully contracted, antiserum is completely precipitated, then the antiserum is collected antiserum and centrifuged to obtain a supernatant, and the supernatant is sub-packed for standby use. The method is simple and quick, and the prepared antibody is highly specific and can be used to detect silk fibroin in textiles.

Description

technical field [0001] The invention relates to a preparation method of polyclonal antibodies, in particular to a method for preparing tussah silk fibroin antibodies by using characteristic polypeptides. Background technique [0002] Silk is a kind of natural polymer protein fiber, which is mainly divided into two categories: domestic silkworm silk and wild silkworm silk. Wild silkworms are also called tussah silkworms. They get their name because they like to eat oak leaves. They can reel silk from their cocoons and are mainly used for weaving tussah silk. my country is the first country to use tussah silkworms and raise tussah silkworms, but its origin has always been a hot and difficult research point, and a more scientific method is urgently needed to identify silk fabrics in early tombs or remains. In the past, there were many methods of identifying ancient silk fabrics. However, as an organic polymer material, silk has been in the environment of tombs or ruins for a l...

AI Technical Summary

Problems solved by technology

In the past, there were many methods of identifying ancient silk fabrics. However, as an organic polymer material, silk has been in the environment of tombs or ruins for a long time, and it will inevitably be affected by various factors, resulting in problems such as degradation of silk proteins and breakage of macromolecular chains. It is difficult to detect the presence of silk fibroin by some traditional methods

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