Electrode type blood sugar test strip

Abstract

The invention discloses an electrode type blood sugar test strip, which comprises a substrate layer, an electrode layer, an insulating layer, a double-sided adhesive layer and a hydrophilic film layer from bottom to top in order. The electrode layer is provided with a first hematocrit test electrode, a second hematocrit test electrode, a work electrode, a reference electrode, a background electrode, a start electrode and leads for all the electrodes and measuring equipment to form circuit connection, the insulating layer and the double-sided adhesive layer are both provided with gaps exposing the first hematocrit test electrode, the second hematocrit test electrode, the work electrode, the reference electrode and the background electrode, and the hydrophilic film layer covers the gaps. The reference electrode and the background electrode exposed by the gaps are coated with water-based ink containing electron-transferring medium through silk screen printing, the work electrode exposed by the gap is coated with water-based ink containing the electron-transferring medium and FAD-GDH, and the electron-transferring medium is methyl methacrylate-N-vinyl pyrrolidone-vinyl ferrocene polymer or hexaammineruthenium chloride.

Description

technical field [0001] The invention belongs to the technical field of blood sugar detection, and in particular relates to an electrode type blood sugar test strip. Background technique [0002] In order to meet the growing needs of diabetic patients for daily monitoring of glucose levels in the blood, a large number of glucose testing instruments have appeared on the market, most of which use electrochemical methods, when enzymes catalyze the glucose reaction in the blood After the instantaneous electrons are captured by the medium, they are transferred to the electrodes under the action of voltage to form a current loop. The blood glucose meter detects the current signal and converts it into a glucose concentration reading for display. [0003] At present, most blood glucose electrodes on the market use the two-electrode or three-electrode method to test the blood glucose concentration. These electrodes are usually affected by other interfering substances in the sample dur...

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Problems solved by technology

[0003] At present, most blood glucose electrodes on the market use the two-electrode or three-electrode method to test the blood glucose concentration. These electrodes are usually affected by other interfering substances in the sample during the test, and are also greatly affected by the volumetric ratio of the blood. Large, greatly affecting the accuracy of the test results

In terms of enzyme selection, most blood glucose electrodes use glucose oxidase, which will be seriously affected by oxygen in the air or blood oxygen partial pressure, resulting in deviations in the results of fingertip blood and venous blood tests

In terms of medium selection, most blood glucose electrodes use potassium ferricyanide or ferrocene and its derivatives. These two media have technical defects. Among them, potassium ferricyanide has poor transfer efficiency and requires a large amount of reagents. Addition is not only very disturbed by reducing substances in the blood, such as uric acid, ascorbic acid, acetic acid aminophenol, etc., but also affects the activity of enzymes and the physical properties of reagents, affecting the repeatability of test results; ferrocene and its derivatives Its water solubility is very poor. In order to add it to the reagent, it is necessary to increase the complexity of the production process, and due to the poor solubility, the amount of addition is very limited, which limits the test linearity and test range of the reagent. Some products are dissolved and added with organic reagents. It will also affect the physical properties of the reagent and affect the activity of the enzyme, affecting the repeatability of the test results

Moreover, most of the blood glucose electrodes on the market are greatly affected by temperature, which is partly compensated by compensating the test data through the machine test environment temperature. However, due to the difference between the temperature of the sample on the electrode and the ambient temperature, the compensation result cannot be fully compensated. Bias in test data

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