Preparation method for chitosan porous hemostatic sponge

A technology of hemostatic sponge and chitosan, which is used in medical science, bandages, absorbent pads, etc., can solve problems such as increasing insecurity, and achieve the effects of good antibacterial effect, uniform pore size and high content

Active Publication Date: 2016-05-04
苏州和其美生物材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, acetic acid is highly irritating to the human body, and its use as a dissolving agent increases the unsafe use of materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, a kind of preparation method of chitosan porous hemostatic sponge, comprises the following steps:

[0022] Step 1, the preparation of solution:

[0023] 1) To prepare a chitosan solution, weigh ascorbic acid and add it to distilled water, fully stir and dissolve it in a water bath at 40°C-50°C to obtain a 0.05mol / L-0.3mol / L ascorbic acid solution; measure acetic acid and add it to 100ml Constant volume in a volumetric flask to prepare an acetic acid solution with a concentration of 0.1% to 0.5% by volume; weigh chitosan and add it to the ascorbic acid solution, after mixing thoroughly, add the acetic acid solution, and place in a water bath at 40°C to 50°C Stir evenly to obtain a chitosan solution with a mass percent concentration of 5% to 8%, which is stored for use, wherein the added acetic acid solution accounts for 0.2% to 0.5% of the total solution;

[0024] 2) Prepare a gelatin solution, weigh the gelatin and add it to distilled water, fully dissolv...

Embodiment 2

[0030] Embodiment 2, a kind of preparation method of chitosan porous hemostatic sponge, comprises the following steps:

[0031] Step 1 is consistent with embodiment 1;

[0032] Step 2, the preparation of chitosan porous hemostatic sponge:

[0033] Get described chitosan solution, gelatin solution and glycerin solution according to the volume ratio of 3: 3: 1 and fully stir and mix in 40 ℃ of water bath conditions, obtain mixed milky white gel liquid, then get described calcium chloride solution and add, in Fully stir for 50 minutes in a water bath at 40°C, then add the tannic acid solution, and continue stirring for 40 minutes in a water bath at 40°C to obtain a mixed red gel. The calcium chloride solution and the tannic acid solution account for 2% and 0.05% respectively; the mixed red gel solution is taken, poured into a mold, and pre-frozen at -50°C for 12 hours to form an ice cube, and then Chitosan porous hemostatic sponge was obtained after vacuum freeze-drying for 60 ...

Embodiment 3

[0034] Embodiment 3,, a kind of preparation method of chitosan porous hemostatic sponge, comprises the following steps:

[0035] Step 1 is consistent with embodiment 1;

[0036] Step 2, the preparation of chitosan porous hemostatic sponge:

[0037] Get described chitosan solution, gelatin solution and glycerol solution according to the volume ratio of 3: 3: 1 and fully stir and mix in 50 ℃ of water bath conditions, obtain mixed milky white gel liquid, then get described calcium chloride solution and add, in Fully stir for 30 minutes in a water bath at 50°C, then add the tannic acid solution, and continue stirring for 20 minutes in a water bath at 50°C to obtain a mixed red gel. The calcium chloride solution and the tannic acid solution accounted for 0.4% and 0.1% respectively; the mixed red gel solution was taken, poured into a mold, and pre-frozen at -80°C for 12 hours to form an ice cube, and then Chitosan porous hemostatic sponge was obtained after vacuum freeze-drying fo...

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PUM

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Abstract

The invention discloses a preparation method for a chitosan porous hemostatic sponge, which includes the following steps: (Step 1) preparation of solutions; (Step 2) preparation of the chitosan porous hemostatic sponge: the chitosan solution, the gelatin solution and the glycerine solution are sufficiently agitated to be mixed according to the volume ratio of 3:3:1 under the water bath condition of 25DEG C, so that a mixed milky gel solution is obtained, the gel solution is then added with a calcium chloride solution, sufficiently agitated under the water bath condition of 25DEG C for 30 minutes and then added with a tannin solution and continues to be agitated under the water bath condition of 25DEG C for 20 minutes, so that a mixed red gel solution is obtained, and the calcium chloride solution and the tannin solution in the obtained mixed red gel solution respectively account for 0.5 percent and 0.01 percent; after the mixed red gel solution is poured into a mold, the mixed red gel solution is prefrozen for more than 12 hours to form an ice block and then freeze-dried under vacuum for 48 hours, the chitosan porous hemostatic sponge is obtained. The preparation method has the following advantages: preparation is simple, and the finished product has high chitosan content, high staunching speed and good antibacterial effect, and can naturally degrade.

Description

technical field [0001] The invention belongs to the field of preparation of medical hemostatic materials, in particular to a preparation method of chitosan porous hemostatic sponge. Background technique [0002] Over the years, there have been more and more studies on hemostatic materials, most of which are natural products that can be degraded in vivo, such as fibrin glue, powdered collagen, gelatin sponge, but these materials have certain shortcomings, the source of fibrin glue In the blood plasma of humans or mammals, it is limited to local application, which may infect blood-borne diseases of humans or animals; collagen, as a foreign body, may cause wound fever and allergies; gelatin sponge has poor mechanical properties and is easy to break. There are also some hemostatic materials imported from abroad, such as instant gauze, which have a good hemostatic effect but are expensive, which limits their application range. Therefore, it is very important to have a hemostatic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/42
CPCA61L15/28A61L15/425C08L5/08
Inventor 蓝广芊卢必涛代方银陈景浩余堃刘佳伟
Owner 苏州和其美生物材料有限公司
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