Mir-106b-5p, detection primer thereof and application of antisense nucleotide sequence thereof in fields of ischemic cerebral stroke diagnosis and treatment

A technology of mir-106b-5p and 1.mir-106b-5p, which is applied in the application field of microRNA, can solve the problems of small time window of thrombolytic therapy, reperfusion injury, aggravated excitotoxicity, etc. The effect of reducing cerebral infarction size, good diagnostic sensitivity and specificity, and improving neurological function score

Inactive Publication Date: 2016-05-04
JIANGSU PROVINCIAL HOSPITAL OF TCM
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first-line drug for the clinical treatment of stroke is thrombolytic drug, but thrombolytic therapy has a series of shortcomings such as small treatment time window and serious reperfusion injury, and it has been reported to destroy the blood-cerebrospinal fluid barrier, increase the risk of bleeding, and exacerbate the excitatory neuropathy. Toxicity and other serious adverse reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mir-106b-5p, detection primer thereof and application of antisense nucleotide sequence thereof in fields of ischemic cerebral stroke diagnosis and treatment
  • Mir-106b-5p, detection primer thereof and application of antisense nucleotide sequence thereof in fields of ischemic cerebral stroke diagnosis and treatment
  • Mir-106b-5p, detection primer thereof and application of antisense nucleotide sequence thereof in fields of ischemic cerebral stroke diagnosis and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Diagnostic value of miR-106b-5p in acute ischemic stroke

[0034] Step 1: Preliminary isolation of miRNAs in peripheral blood of acute ischemic stroke:

[0035] 1. Patients with initial acute IS group: All patients were diagnosed in accordance with the diagnostic criteria for acute stroke revised by the 4th National Cerebrovascular Disease Academic Conference in 1996, and were confirmed by head CT or MRI at the same time, and the onset time did not exceed 24 hours. Exclusion criteria: combined with disturbance of consciousness or mental symptoms; severe heart, liver, kidney, and pulmonary insufficiency; history of trauma and surgery; history of cancer. Within 24 hours of the onset, the patients were tested for fasting blood glucose, blood routine, urine routine, complete biochemical items, glycosylated hemoglobin, six coagulation items, and C-reactive protein. Another healthy person (negative control, NC) was selected as the normal control group. Informed consent was ...

Embodiment 2

[0045] Neuroprotective effect of miR-106b-5p antisense nucleotide antagomir miR-106b-5p on focal cerebral ischemia-reperfusion injury:

[0046] 1. Grouping and treatment of experimental animals: SPF grade (referring to specific pathogen-free experimental animals) 8-10 week old male SD rats (provided by Beijing Weitong Lihua Company), body weight (280-330g). Experimental grouping: 4 groups in total, 6 rats in each group, 24 rats in total. (1) Sham operation group: only blood vessels and nerves were separated, and no suture was inserted. (2) MCAO model untreated group: After successful modeling, the rats were not given any intervention. (3) AntagomirmiR-106b-5p treatment group: AntagomirmiR-106b-5p50nmol / kg was injected into the tail vein before reperfusion. The sequence of antagomirmiR-106b-5p is (SEQ ID NO: 5): 5'-AUCUGCACUGUCAGCACUUUA-3', here is the miR-106b-5p antisense nucleotide sequence, that is, the nucleotide sequence injected into rats can interfere with miR -106b-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses miR-106b-5p, a detection primer thereof and application of the antisense nucleotide sequence thereof in the fields of ischemic cerebral stroke diagnosis and treatment, and belongs to the field of application of micro ribonucleic acid. The sequence of a reverse transcription primer in the detection primer of the miR-106b-5p is 5'-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGATCTGCAC-3'. The antisense nucleotide sequence of the miR-106b-5p is 5'-AUCUGCACUGUCAGCACUUUA-3'. The miR-106b-5p, the detection primer thereof and the antisense nucleotide sequence thereof are associated with acute ischemic cerebral strokes, the miR-106b-5p can become an auxiliary index of acute ischemic cerebral stroke clinical detection, and a therapeutic target is provided for prevention of the disease. The miR-106b-5p has the advantages of being good in treatment effect, easy to operate, free of toxic and side effects and the like.

Description

technical field [0001] The invention belongs to the application field of microribonucleic acid, and more specifically relates to the application of detection primers and antisense nucleotide sequences of miR-106b-5p in the preparation of drugs for treating ischemic stroke. Background technique [0002] Stroke (stroke) belongs to the category of "stroke" in traditional Chinese medicine. It is a common, frequently-occurring, and refractory disease that occurs frequently in middle-aged and elderly people. Its disability rate, mortality rate, and recurrence rate are still high. According to a report in 2013, stroke has become the leading cause of death in China. The most common type of stroke is ischemic stroke. At present, the diagnosis of ischemic stroke mainly relies on the clinical observation and imaging evidence of the attending physician. In the early stage of ischemic stroke, the clinical manifestations are not always typical, and the image changes of computed tomograp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K31/7088A61K48/00A61P9/10
CPCC12Q1/6883A61K31/7088C12Q2600/158C12Q2600/178
Inventor 张春兵李鹏飞高峰滕凤猛
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap