Method for discriminating acacia honey and rape honey on basis of H-nuclear magnetic resonance

A nuclear magnetic resonance and nuclear magnetic resonance spectrometer technology, applied in the direction of nuclear magnetic resonance analysis, etc., can solve the problems of strong market demand, low output of acacia honey, high price, etc., to avoid subjective factors, simple and easy to operate, and accurate and reliable methods Effect

Inactive Publication Date: 2016-05-04
WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The output of acacia honey is low, but the m

Method used

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  • Method for discriminating acacia honey and rape honey on basis of H-nuclear magnetic resonance
  • Method for discriminating acacia honey and rape honey on basis of H-nuclear magnetic resonance
  • Method for discriminating acacia honey and rape honey on basis of H-nuclear magnetic resonance

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Experimental program
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Embodiment Construction

[0036] 1. Instruments, reagents and samples

[0037] 1.1 Instruments:

[0038] 500MHz liquid nuclear magnetic resonance spectrometer (BrukerAVANCE), Switzerland BrukerBiospin Company; micro benchtop centrifuge (SorvallLegendMicro17R), American ThermoScientific Company; electronic balance (AL104), Mettler-Toledo Instrument (Shanghai) Co., Ltd.; 5mm NMR tube, United States WILMAD

[0039] 1.2 Reagents:

[0040] K 2 HPO 4 ·3H 2 O and NaH 2 PO 4 2H 2 O (analytical grade) was purchased from Shanghai Sinopharm Group Reagent Co., Ltd. NaN 3 (analytical pure) was purchased from Tianjin Fuchen Chemical Reagent Factory. Heavy water (D 2 O, 99.9% deuterated, containing sodium 2,2,3,3-deuterated trimethylsilyl propionate (TSP), 0.05 wt%) was purchased from Sigam-Aldrich Company. Phosphate buffered saline (K 2 HPO 4 :NaH 2 PO 4 =4:1, 0.15mol / L, pH7.40) use 10% D 2 O's double distilled water preparation. Wherein the buffer solution contains TSP and NaN 3 (0.01%, w / v) were...

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Abstract

The invention discloses a method for discriminating acacia honey and rape honey on the basis of H-nuclear magnetic resonance, belongs to the field of food detection, and relates to an application of H-nuclear magnetic resonance fingerprint in food detection. The method comprises the following steps: respectively establishing the H-nuclear magnetic resonance database of the rape honey and the H-nuclear magnetic resonance database of the acacia honey, constructing a discriminating model by using combination of a factor analysis technology and a discriminant analysis technology, discriminating a honey sample to be detected, and determining the honey to be discriminated as the rape honey or the acacia honey. The method has the advantages of reliable technology, simple operation, treatment of a large amount of samples in a short time, rapid discrimination of the kind of honey, avoiding of subjective factors and personal errors, and scientific and reliable conclusion.

Description

technical field [0001] The invention relates to food detection, and more specifically relates to a method for identifying acacia honey and rapeseed honey by using hydrogen nuclear magnetic resonance fingerprints. Background technique [0002] Honey is a natural sweet substance that is fully brewed after bees collect nectar, secretions or honeydew from plants, mix with their own secretions (national standard for honey GB14963-2011GB). As a natural health care product and nutritional product, it is favored by consumers. Honey is usually classified according to the source plants, such as rapeseed honey, acacia honey, jujube honey, twig honey and so on. The honey production of different nectar source plants is high or low, and there are obvious differences in price. Driven by profit, some unscrupulous manufacturers will shoddy and confuse real ones with fake ones. Therefore, there is a need to develop efficient and reliable methods to identify honeys of different botanical or...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 陈雷刘红兵罗立廷
Owner WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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