A method for measuring alkaloid release in mainstream cigarette smoke
A technology of mainstream smoke and alkaloids of cigarettes, applied in the direction of measuring devices, instruments, scientific instruments, etc.
Active Publication Date: 2020-06-16
CHINA TOBACCO GUIZHOU IND
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Problems solved by technology
In recent years, new detectors have been developed and applied to the determination of tobacco alkaloids in tobacco leaves and shredded tobacco, such as nitrogen chemiluminescence detectors, tandem mass spectrometry, etc., but there are few reports on the application in mainstream cigarette smoke
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Embodiment 1
[0070] A method for simultaneously measuring the release of eight alkaloids in mainstream cigarette smoke, comprising the steps of:
[0071] Step 1: Prepare internal standard solution:
[0072] Weigh anthracene-d 10 0.05g, accurate to 0.0001g, put in a 50mL volumetric flask, add methanol to constant volume, and obtain the internal standard solution;
[0073] Step 2: Prepare alkaloid mixed standard solution:
[0074] (1) Weigh 0.1023g nicotine, 0.0112g nornicotine, 0.02g mesmin, 0.01g diene nicotine, 0.01g anatabine, 0.01g anatabine, 0.0276g cotinine, 0.01 g 2,3'-Bipyridine 8 kinds of alkaloid standard samples of different quality, accurate to 0.0001g, put them in 10mL volumetric flask respectively, add dichloromethane to constant volume, shake well, and get 8 standard solutions;
[0075] (2) Pipette 10, 20, 50, 100, 200, and 500 μL of each standard solution into six 10mL volumetric flasks, add 100 μL of internal standard solution, dilute to volume with ethyl acetate, and sh...
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Abstract
The invention relates to a method for measuring the released alkaloid amount in the mainstream smoke of cigarette. The method comprises the following steps: (1) preparing an alkaloid standard solution; (2) preparing a solution of a sample to be tested; (3) analyzing the alkaloid standard solution by GC-MS / MS, taking the ratio of the peak areas of the alkaloid and an internal standard substance as the vertical coordinate, taking the concentration of the alkaloid mixed standard solution as the horizontal coordinate, and carrying out regression analysis to obtain a regression equation and related coefficient; (4) analyzing the solution of the sample to be tested by GC-MS / MS, and calculating the released alkaloid amount according to the regression equation. The method has the advantages that the alkaloids in the smoke of cigarette can be analyzed and detected at the same time, the pretreatment is simple, the detection result is accurate, the repeatability is good, the sensitivity is high, and the method can be used to measure alkaloid in the smoke of cigarette and other complicated matrix.
Description
technical field [0001] The invention belongs to the technical field of tobacco detection, and in particular relates to a method for measuring the release amount of alkaloids in mainstream smoke of cigarettes. Background technique [0002] Alkaloids are a class of tobacco alkaloids in mainstream cigarette smoke that directly affect the chemical properties and sensory characteristics of smoke, mainly including nicotine, nornicotine, myosmine, and diene nicotine. (nicotyrine), anabasine, anatabine, cotinine, 2,3'-bipyridyl (2,3'-dipyridyl), etc., which are derived from alkaloids in shredded tobacco Direct transfer and pyrolysis of cut tobacco, so accurate determination of alkaloid release in mainstream smoke of cigarettes plays an important role in the evaluation of smoking quality of cigarettes. [0003] The currently reported analytical methods for tobacco alkaloids mainly include gas chromatography, gas chromatography-mass spectrometry, high performance liquid chromatograph...
Claims
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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 姬厚伟刘剑彭黔荣张丽王芳刘纳纳满杰韩伟
Owner CHINA TOBACCO GUIZHOU IND