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Bird flu virus detection reagent kit

A bird flu virus and detection kit technology, applied in the field of medical detection, can solve the problems of complicated operation requirements, infectivity, and inability to detect the virus early, and achieve the effect of enhanced color rendering and rapid detection

Inactive Publication Date: 2016-05-04
王好玉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each method has certain defects. For example, although the detection technology of fluorescent quantitative PCR is the most sensitive, it is costly and requires complicated operation, so it is not easy to popularize.
Colloidal gold detection reagents have disadvantages such as low sensitivity
The results of AGP, HI, and virus neutralization are accurate, but they all need to prepare purified, concentrated and complete viruses as detection antigens, and avian influenza virus is a highly pathogenic virus, which needs to be completed in a P3 laboratory (biosafety protection level 3 laboratory) ), it is not easy to produce, purify, and cost is high, and there is infectivity and easy to disperse poison, so there are great limitations in application
In addition, the body is infected with avian influenza virus, and protective antibodies begin to appear after the 10th day, and the detection of antibodies cannot achieve the purpose of early detection of the virus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A detection kit for avian influenza virus, comprising avian influenza virus envelope antigen, a detection probe for rabbit anti-chicken IgY antibody labeled with nano-gold, and a dotted gold immune centralized diafiltration device. The centralized diafiltration device consists of a small plastic box, a point It consists of three parts: a nitrocellulose membrane added with avian influenza virus envelope antigen and a water-absorbing pad. There is a square hole with a side length of about 0.6 cm in the center of the lid of the small plastic box. The corresponding square groove is as big as the square hole on the box cover; the absorbent pad is placed in the groove of the box, and the nitrocellulose membrane is placed between the absorbent pad and the box cover, and the area of ​​the absorbent pad is smaller than that of the nitrocellulose membrane. 1 / 3 smaller; the concentration of the avian influenza virus envelope antigen is 1.75 μg / mL; the concentration of the nano-gold...

Embodiment 2

[0028]A detection kit for avian influenza virus, comprising avian influenza virus envelope antigen, a detection probe for rabbit anti-chicken IgY antibody labeled with nano-gold, and a dotted gold immune centralized diafiltration device. The centralized diafiltration device consists of a small plastic box, a spot It consists of three parts: a nitrocellulose membrane added with avian influenza virus envelope antigen and a water-absorbing pad. There is a square hole with a side length of about 0.8 cm in the center of the lid of the small plastic box. The corresponding square groove is as big as the square hole on the box cover; the absorbent pad is placed in the groove of the box, and the nitrocellulose membrane is placed between the absorbent pad and the box cover, and the area of ​​the absorbent pad is smaller than that of the nitrocellulose membrane. Small 5 / 12; the concentration of the avian influenza virus envelope antigen is 1.80 μg / mL; the concentration of the nano-gold-la...

Embodiment 3

[0037] A detection kit for avian influenza virus, comprising avian influenza virus envelope antigen, a detection probe for rabbit anti-chicken IgY antibody labeled with nano-gold, and a dotted gold immune centralized diafiltration device. The centralized diafiltration device consists of a small plastic box, a spot It consists of three parts: a nitrocellulose membrane added with bird flu virus envelope antigen and a water-absorbing pad. There is a square hole with a side length of about 1.0 cm in the center of the lid of the small plastic box. The corresponding square groove is as big as the square hole on the box cover; the absorbent pad is placed in the groove of the box, and the nitrocellulose membrane is placed between the absorbent pad and the box cover, and the area of ​​the absorbent pad is smaller than that of the nitrocellulose membrane. 1 / 2 smaller; the concentration of the avian influenza virus envelope antigen is 1.85 μg / mL; the concentration of the nano-gold-labeled...

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PUM

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Abstract

The invention discloses a bird flu virus detection reagent kit which comprises a bird flu virus cyst membrane antigen, a nanogold-labeled rabbit anti-chicken IgY antibody detection probe, and a dot immunogold concentrated diafiltration device. The concentrated diafiltration device is composed of a small plastic box, a nitrocellulose membrane where the bird flu virus cyst membrane antigen is added dropwise, and a water-absorbing pad. The water-absorbing pad is placed in a groove of the small plastic box, and the nitrocellulose membrane is placed between the water-absorbing pad and a kit cover. On the basis that a bird flu virus cyst membrane has the antigen characteristic, the nitrocellulose membrane of the bird flu virus cyst membrane antigen is prepared, then a nanogold-labeled rabbit anti-chicken IgY antibody serves as the detection probe, the dot immunogold concentrated diafiltration method is used for detection, detection speed is high, sensitivity is high, and the whole detection reagent kit is easy to prepare and low in cost.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to an avian influenza virus detection kit. Background technique [0002] Avian influenza is an acute infectious disease of poultry caused by avian influenza virus, and some subtypes of avian influenza virus can also infect humans. Avian influenza viruses can be divided into 16 HA subtypes and 9 NA subtypes according to the differences in the antigenic relationship between their surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). According to its pathogenicity, it can be divided into highly pathogenic avian influenza and low pathogenic avian influenza. Highly pathogenic avian influenza includes H5 and H7 subtypes. Especially in recent years, the H5 subtype avian influenza outbreaks have caused huge economic losses to the poultry industry in many countries and regions. [0003] At present, the methods for detecting avian influenza virus mainly include: fluores...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N2333/11
Inventor 王好玉白雪贾莉
Owner 王好玉
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