Penicillium expansum nucleotide sequence qualitative standard sample and preparing method thereof

A standard sample, penicillium nucleic acid technology, applied in the direction of biochemical equipment and methods, DNA preparation, microbial measurement/inspection, etc., can solve the problems of lack of standardization, traceability awareness and understanding confusion, etc.

Inactive Publication Date: 2016-05-11
刘淑艳 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The status of traceability description of classification and grading national standard samples shows that people's awareness and understanding of the traceability of such standard samples is in a relatively chaotic stage, and there is no unified specification

Method used

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  • Penicillium expansum nucleotide sequence qualitative standard sample and preparing method thereof
  • Penicillium expansum nucleotide sequence qualitative standard sample and preparing method thereof
  • Penicillium expansum nucleotide sequence qualitative standard sample and preparing method thereof

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Embodiment

[0056] Using the theory of classification and grading of standard substances, the establishment of a reference system and reference indicators for the determination and traceability of qualitative standard samples of Penicillium elongatum nucleic acid sequences includes the following steps:

[0057] The first step: the establishment of the reference system for the determination and traceability of reference materials:

[0058] (1) Expand the taxonomic status of Penicillium

[0059] The taxon characteristic value of a Penicillium in the standard sample is Penicillium extensa (species), wherein the characteristic used for classification is the taxon status of Penicillium, and the characteristic value of the Penicillium (tested object) is Penicillium extensa The taxonomic status characteristic value set of mold (species) and Penicillium (genus) includes 4 subgenus-level subsets, namely, Aspergillus subgenus, Fork-like subgenus, Penicillium subgenus, and Birotina subgenus, while P...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a penicillium expansum nucleotide sequence qualitative standard sample and a preparing method thereof. Penicillium expansum in the logarithmic phase is acquired, total DNA extraction is conducted, specific PCR amplification sequencing is conducted, and then plasmid preparation is conducted. The prepared nucleotide sequence qualitative standard sample does not have biological activity any more, biological pollution and infection are avoided, the source of molecular biology positive control is available, and the sample is stable, free of pollution and easy to store. Based on the fact that penicillium expansum has the nucleotide sequence technical index with taxonomical significance, the problems of penicillium expansum nucleotide sequence standard sample source tracing reference system and reference index are solved, the penicillium expansum nucleotide sequence standard sample with real source tracing significance is obtained through application in setting value analysis of the standard sample, and the penicillium expansum nucleotide sequence qualitative standard sample has great significance in development of nucleotide sequence classified gradation standard samples in China.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a qualitative standard sample of Penicillium elongatus nucleic acid sequence and a preparation method thereof. Background technique [0002] The World Food and Agriculture Organization (FAO) reports that about 25% of the world's crops are polluted by fungi and their mycotoxins every year, and about 2% of the crops lose their nutritional value and economic value due to serious pollution. Direct and indirect losses caused by pollution may reach tens of billions of dollars. [0003] PatμLin, also known as patulin, has a chemical name of 4-hydroxy-4H-fur[3,2c]pyran-2(6H)-one, and is a member of Penicillium, Aspergillus and A secondary metabolite of various fungi such as Byssochlamys. In the 1950s, it was found that patulin not only pollutes a large amount of grain and feed, but also seriously pollutes apples and their products. Studies have proved that patulin is a toxin that can caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/63C12N15/10
CPCC12Q1/6895C12Q2600/166
Inventor 刘淑艳蒋丹孙晓飞颜怀玉郑江
Owner 刘淑艳
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