Fusarium oxysporum nucleotide sequence qualitative standard sample and preparing method thereof

A technology of Fusarium oxysporum and nucleic acid sequence, which is applied in biochemical equipment and methods, DNA preparation, microbial measurement/inspection, etc., and can solve the problems of traceability awareness and understanding confusion, lack of norms, etc.

Inactive Publication Date: 2016-05-11
蒋丹 +3
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The status of traceability description of classification and grading national standard samples shows that people's awareness and

Method used

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  • Fusarium oxysporum nucleotide sequence qualitative standard sample and preparing method thereof
  • Fusarium oxysporum nucleotide sequence qualitative standard sample and preparing method thereof
  • Fusarium oxysporum nucleotide sequence qualitative standard sample and preparing method thereof

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Embodiment

[0055] Using the classification and grading theory of standard substances, establish a reference system and reference indicators for the determination and traceability of qualitative standard samples of Fusarium oxysporum nucleic acid sequences, including the following steps:

[0056] The first step: the establishment of the reference system for the determination and traceability of reference materials:

[0057] (1) Taxonomic status of Fusarium oxysporum

[0058] The taxon property value of a Fusarium oxysporum in a contaminated soy flour sample is Fusarium oxysporum (species). Among them, the characteristic used for classification is the taxonomic status of Fusarium oxysporum, and the characteristic value of the Fusarium oxysporum (measured object) is Fusarium oxysporum (species). The taxonomic status characteristic value set of Fusarium (genus) includes Orthospora group, Lyser group, Paleomyces group, Cladosporium group, Brick red group, Meili group, Matt group, spider silk...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a fusarium oxysporum nucleotide sequence qualitative standard sample and a preparing method thereof. Fusarium oxysporum in the logarithmic phase is acquired, total DNA extraction is conducted, specific PCR amplification sequencing is conducted, and then plasmid preparation is conducted. The prepared nucleotide sequence standard sample does not have biological activity any more, biological pollution and infection are avoided, the source of molecular biology positive control is available, and the sample is stable, free of pollution and easy to store. Based on the fact that fusarium oxysporum has the nucleotide sequence technical index with taxonomical significance, the problems of fusarium oxysporum nucleotide sequence standard sample source tracing reference system and reference index are solved, the fusarium oxysporum nucleotide sequence standard sample with real source tracing significance is obtained through application in setting value analysis of the standard sample, and the fusarium oxysporum nucleotide sequence qualitative standard sample has great significance in development of nucleotide sequence classified gradation standard samples in China.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to a qualitative standard sample of nucleic acid sequence of Fusarium oxysporum and a preparation method thereof. Background technique [0002] The World Food and Agriculture Organization (FAO) reports that about 25% of the world's crops are polluted by fungi and their mycotoxins every year, and about 2% of the crops lose their nutritional value and economic value due to serious pollution. Direct and indirect losses caused by pollution may reach tens of billions of dollars. [0003] Among the currently known mycotoxins, Fusarium (Fusarium) is an important plant pathogen that harms crops such as wheat, corn, rice, and vegetables, causing severe production reduction. The zearalenone toxin produced by Fusarium toxin It has obvious cytotoxicity and inhibitory effect on protein and DNA synthesis, and has certain teratogenicity and mutagenicity, and the pollution is very extensive. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/63C12N15/10
CPCC12Q1/6895C12Q2600/166
Inventor 蒋丹刘淑艳丁健富瑶郑江
Owner 蒋丹
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