A method and device for continuous and automatic reading of multiple biochip transmission signals

Abstract

The invention provides a method of continuously automatically reading transmission signal values of multiple developing microarray biochips, and a reading instrument and a reading system for realizing the method. The reading method is characterized by comprising: (1) scanning a glass chip bar code to obtain chip bar code information; (2) placing the main camera used for shooting a chip image and the background light source used for main camera lighting on two sides of a glass chip; (3) identifying parameters according to the bar code information, chip images and signal points, automatically identifying and positioning the signal points, and automatically obtaining signal point coordinates; (4) automatically obtaining signal values of the signal points according to the obtained signal point coordinates; and (5) automatically continuously reading a plurality of chips (which can be chips of different types) through position movement.

Description

technical field [0001] The present invention relates to a biochip reading method, a reading instrument and a reading system for realizing the method, in particular to a method for continuously and automatically reading multiple transmission signal values ​​of color-developing microarray biochips, A reading instrument and a reading system for realizing the method. Background technique [0002] Microarray biochips synthesize or immobilize a series of polymers, such as oligonucleotides, polypeptides, and antibodies, in a lattice arrangement on glass substrates, silicon substrates, or polymer material substrates. These polymers can be labeled with fluorescent or chemichromic markers for optical detection. Chromogenic microarray chips usually use chemical chromogenic markers such as digoxin, horseradish peroxidase, and biotin to mark the polymers immobilized on the chip, so that they can be read by a biochip reader. Response result information. The chromogenic microarray bioch...

AI Technical Summary

Problems solved by technology

[0004] Dust, fiber, background and other pollutants with a certain signal value sometimes exist in the area around the array point of the chromogenic microarray biochip, which will interfere with the coordinate positioning of the signal point and the accurate collection of signal value

The current chromogenic microarray biochip reader usually needs to manually confirm the type of the chip, and then manually adjust it in the computer software to find the correct array point, which has a low degree of automation and low efficiency; when multiple types of biochips need to be read When using biochips, the efficiency of manual reading will be lower, and there may be mistakes in manually confirming the chip type due to the increase in work intensity, which will lead to a decrease in the accuracy of chip reading results; the collected signal values ​​​​are often due to the above-mentioned chip Pollutants and manual operation lead to interference and distortion; in the chip signal collection link, usually the light passes through the chip and then shines on the backlight board, and the backlight board reflects the chip signal and then is collected, and the dust accumulated on the backlight board affects the signal value. The interference is very large, and it will also cause the collected signal to be distorted; one chip is detected at a time, and the detection throughput is low

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