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Method for identifying honey syrup adulteration based on oligosaccharide isomer composition difference

A technology of isomers and honey, applied in the field of detection, can solve the problems of dependence on standards, loss of information, long analysis time, etc., and achieve good repeatability

Inactive Publication Date: 2020-02-11
NANCHANG UNIV
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  • Abstract
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Problems solved by technology

[0003] Nowadays, the methods commonly used in honey syrup adulteration detection mainly include stable carbon isotope method, nuclear magnetic resonance method, chromatography, spectroscopy and mass spectrometry. Standards and other issues

Method used

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  • Method for identifying honey syrup adulteration based on oligosaccharide isomer composition difference
  • Method for identifying honey syrup adulteration based on oligosaccharide isomer composition difference
  • Method for identifying honey syrup adulteration based on oligosaccharide isomer composition difference

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Embodiment 1

[0024] The mass spectrometer used in the example of the present invention is a matrix-assisted laser desorption ionization mass spectrometer from Bruker, Germany, and the data processing system is the FlexAnalysis data processing system from Bruker, USA.

[0025] In this experiment, MALDI-MS was used for mass spectrometry detection of honey, syrup and adulterated honey. like figure 1 As shown, NEDC, as a new type of organic matrix, can make oligosaccharides produce strong [M+Cl] - ions with little background interference in the small molecule region. Peaks at m / z 215.0, 377.1, 539.1, and 701.2 were analyzed by MALDI-Q-FTICR and determined to be chloride ion adducts of monosaccharides, disaccharides, trisaccharides, and tetrasaccharides based on accurate molecular weights (see Table 1). In LIFT-TOF / TOF mode, [M+CL] - First drop HCL to form a hydrogen anion [M-H] - , and then dissociated into smaller fragment ions, including some cross-ring fragments, such as [M-H-120] - ,...

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Abstract

The invention aims to provide the detection method for identifying honey adulteration based on oligosaccharide isomer composition difference. A complex sample pretreatment process is not needed, MALDIis directly used for collecting mass spectrum data after dissolution and spotting, and adulterated honey added with 10% CS and IS can be rapidly distinguished. The detection method is used for detecting honey, syrup and adulterated honey samples based on a matrix-assisted laser desorption ionization mass spectrometer. According to the method, all samples are not pretreated, and MALDI-MS is directly used for collecting mass spectrometric data after dissolution and spotting; high-throughput analysis can be achieved, and the whole process from sample preparation to data acquisition can be completed within 10min; and moreover, a high-resolution sample spectrogram with good repeatability can be obtained without depending on a sugar standard substance and derivatization.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for identifying adulteration of honey syrup based on differences in composition of oligosaccharide isomers Background technique [0002] Honey (honey) is a kind of natural sweet substance brewed by bees after collecting plant nectar or its secretions, and processing their own enzymes and special substances in the body. It should not contain any exogenous substances or food additives . The demand for honey is increasing due to its high nutritional value, healing properties and unique flavor. Therefore, unscrupulous merchants mix various cheap sweeteners into honey in pursuit of commercial profits. The most common adulterated syrups include corn syrup (CS), high fructose corn syrup (HFCS), and invert syrup (IS). Honey is now the most adulterated type of food after olive oil and milk. [0003] Nowadays, the methods commonly used in honey syrup adulteration detection ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 罗丽萍屈亮亮黄学勇郭夏丽郝影宾崔萌
Owner NANCHANG UNIV
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