Composition for treating colon cancer and application thereof

A composition and colon cancer technology, applied in the direction of drug combination, medical preparations containing active ingredients, drug delivery, etc., to achieve good application prospects

Inactive Publication Date: 2016-06-01
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no report about the specific combination of cottonone-sodium taurate and chloroquine for the treatment of colon ...

Method used

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  • Composition for treating colon cancer and application thereof
  • Composition for treating colon cancer and application thereof
  • Composition for treating colon cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Cell viability (MTS method)

[0030] Spread SW480, SW620, LOVO, Caco-2, CT26, HCT116 and HT29 cells (the cells were all purchased from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences) in a 96-well plate at a density of 3000 cells / well or 5000 cells / well After 24 hours, the cells were completely attached to the wall, and the McCoy'5a (Gibco company) medium (for mother liquor of cottonone-sodium taurate) dissolved with 0, 5, 10, 20, 40, 80 μM cottonone-sodium taurine DMSO dissolved) to replace the old medium, at 37 ° C, 5% CO 2 Cultivate in the incubator for 72 hours, replace the drug medium with McCoy's5a medium (Gibco company) containing 3% (volume percentage) serum, add 20 μL MTS (Promega company) to each well, incubate at 37 ° C for 1 h, shake well at room temperature for 15 min , use a microplate reader (ThermoScientific) to detect the absorbance at 490nm, and use the software GraphPadPrism5.01 to calculat...

Embodiment 2

[0032] Example 2 Tumor Growth Inhibition Experiment in Tumor-bearing Mice

[0033] 1. Drug preparation

[0034] Weigh 0.19296g (0.00024mol) of cottonone-sodium taurine powder and completely dissolve it in 16ml of 10% ethanol solution (in the ethanol solution, the volume ratio of ethanol:water=10:90) as the first solution.

[0035] Meanwhile, 0.00024mol chloroquine (Sigma company) was weighed and completely dissolved in 16ml of 10% ethanol solution as the second solution.

[0036] Then weigh 0.19296 g of cottonone-sodium taurine powder and 0.00024 mol of chloroquine (CQ) and dissolve them in 16 ml of 10% ethanol solution simultaneously as the third solution.

[0037] The above three solutions were equally divided into 1ml / tubes and stored in a -20°C refrigerator.

[0038] 2. Experimental process

[0039] Animals: BALB / c male nude mice aged 6-8 weeks were purchased from Shanghai South Model Animal Center. Nude mice were reared and tested at the Shanghai Southern Model Organi...

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PUM

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Abstract

The invention discloses a composition for treating colon cancer and an application thereof. The composition comprises gossypol ketone-sodium taurate and chloroquine with a mole ratio of 2:1-1:1. The composition is used for preparing the medicines for treating colon cancer. The composition establishes a base for preparing the medicine used for treating colon cancer, and has good application prospect.

Description

technical field [0001] The invention relates to a composition and its application, in particular to a composition for treating colon cancer and its application. Background technique [0002] Gossypol is a phenolic natural compound extracted from cottonseed. In recent years, scientists have conducted in-depth exploration of the anti-tumor mechanism of gossypol. Gossypol can inhibit the growth of various cancer cells, such as prostate cancer and pancreatic cancer. , colon cancer, lung cancer, etc. At the same time, gossypol can also significantly enhance the sensitivity of cancer patients to radiotherapy and chemotherapy in clinical cancer treatment. At present, a series of gossypol derivatives have been reported, such as AT-101, Apogossypol, Tff-37, ABT-737 and WL-276, etc., which have the activity of inhibiting the growth of cancer cells. [0003] However, gossypol has not been used in clinical treatment of cancer, because the hydroxyl group of gossypol brings strong toxic ...

Claims

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Application Information

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IPC IPC(8): A61K31/4706A61K9/08A61P35/00A61K31/255
Inventor 郭方王雪枫姜标王建永严向明
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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