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Method for extracting ursolic acid and 2 alpha-hydroxyl ursolic acid from lagerstroemia speciosa

A technology of hydroxyursolic acid and ursolic acid, applied in the production of steroids, bulk chemicals, organic chemistry, etc., can solve the problems of environmental pollution, low purity and cannot meet the requirements of pharmaceutical grade, etc., and achieve the effect of low pollution

Active Publication Date: 2016-06-01
黑龙江聚川智能科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is: provide a kind of method of extracting ursolic acid and 2a-hydroxy ursolic acid in Lagerstroemia indica, to overcome the prior art can only obtain ursolic acid or 2a-hydroxy ursolic acid from plant raw material alone Problems of ursolic acid and serious environmental pollution; or the problem that although ursolic acid and 2a-hydroxyursolic acid can be obtained at the same time, the purity is low and cannot meet the requirements of pharmaceutical grade

Method used

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  • Method for extracting ursolic acid and 2 alpha-hydroxyl ursolic acid from lagerstroemia speciosa
  • Method for extracting ursolic acid and 2 alpha-hydroxyl ursolic acid from lagerstroemia speciosa

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Embodiment

[0061] Take 1.5kg of minced Lagerstroemia indica raw materials (0.56% ursolic acid content and 0.38% 2a-hydroxyursolic acid content) and add 10L petroleum ether to reflux to extract chlorophyll and oil and other impurities for 10 hours, and recover large The residual petroleum ether in Lagerstroemia indica, the raw materials of Lagerstroemia indica after removing chlorophyll and oil are placed in a supercritical extraction kettle, and soaked in CO 2 In liquid, CO 2 Add entrainer chloroform 75g to the liquid, extract statically for 1 hour, the pressure of the extraction kettle is 22MPa, the temperature is 35°C, and then extract dynamically for 0.5 hour. During dynamic extraction, CO 2 The flow rate is 0.4L / min. Then the liquid in the extraction tank is introduced into the separation tank for separation, the pressure of the control separation tank is 10MPa, the temperature is 50°C, and the separated liquid is exported to obtain the extraction liquid A mainly containing ursolic ...

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Abstract

The invention provides a method for extracting ursolic acid and 2 alpha-hydroxyl ursolic acid from lagerstroemia speciosa. The method comprises the following steps: preparing and cutting a lagerstroemia speciosa raw material; extracting by using petroleum ether; performing supercritical CO2 extraction and separation so as to obtain an extraction liquid containing ursolic acid and an extraction liquid containing 2 alpha-hydroxyl ursolic acid; performing high-speed countercurrent chromatography separation on the extraction liquid containing ursolic acid and the extraction liquid containing 2 alpha-hydroxyl ursolic acid respectively, so as to obtain ursolic acid effluent fraction and 2 alpha-hydroxyl ursolic acid effluent fraction; concentrating respectively, so as to obtain a concentrated ursolic acid liquid and a concentrated 2 alpha-hydroxyl ursolic acid liquid; crystallizing and separating respectively, so as to obtain ursolic acid crystal and 2 alpha-hydroxyl ursolic acid crystal correspondingly; drying, thereby obtaining an ursolic acid medicine and a 2 alpha-hydroxyl ursolic acid medicine.

Description

technical field [0001] The invention belongs to the field of medicinal material purification, and in particular relates to a method for extracting ursolic acid and 2a-hydroxyursolic acid in Lagerstroemia indica. technical background [0002] Lagerstroemia speciosa Pers (Lagerstroemia speciosa Pers), also known as Lagerstroemia speciosa Pers, is a deciduous tree belonging to the genus Lagerstroemiaceae, mainly distributed in the Philippines, Malaysia, Vietnam and other places. Lagerstroemia indica is a folk medicine in Southeast Asian countries, commonly known as "Banaba". The leaves of the plant contain Ursolic Acid, an active substance that lowers blood sugar, C 30 h 48 o 3 , the molecular formula is: [0003] [0004] (I) [0005] and 2a-hydroxyursolic acid, also known as corosolic acid 2-alpha-hydroxyursolic acid, C 30 h 48 o 4 , molecular formula such as: (II) [0006] Therefore, it is very necessary to establish a method to obtain high-purity pharmaceutica...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCY02P20/54
Inventor 冯建光
Owner 黑龙江聚川智能科技有限责任公司
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