Lactobacillus salivarius XJP2 and application thereof
A Lactobacillus salivarius and colony technology, applied in the field of microorganisms, can solve problems such as poor tolerance, achieve good tolerance, fast in vitro culture and reproduction, and prevent bacterial diarrhea.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] Embodiment 1, the screening of Lactobacillus salivarius
[0019] 1. Isolation and screening of Lactobacillus salivarius XJP2
[0020] Taking healthy broiler chickens as samples, scrape the cecal mucosa with sterile glass slides and put it in sterile water, shake and mix well for gradient dilution, pour the MRS medium into the culture and screen the colonies with obvious "calcium dissolution circle", and obtain a single colony after multiple purifications , name it XJP2, and save it. The MRS medium is a general medium for cultivating lactic acid bacteria, and its formula is: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, glucose 20g / L, Tween 801ml / L, dipotassium hydrogen phosphate 2g / L, sodium acetate 5g / L, triammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, calcium carbonate 5g / L, pH6.2.
[0021] The colony morphology of described bacterial strain XJP2 on MRS plate is as follows figure 1 As shown, its biological characteristics: Gr...
Embodiment 2
[0025] Embodiment 2, the growth assay of Lactobacillus salivarius XJP2
[0026] The activated XJP2 bacterial solution was mixed with 1% (about 10 7 cfu / mL) was inoculated in the MRS liquid medium, cultured at 37° C. and 160 rpm for 24 h, sampling every 3 h, measuring the absorbance at 600 nm, and drawing a growth curve. Such as figure 2 As shown, the experimental results show that XJP2 enters the logarithmic growth phase after 3 hours of culture, and enters the stationary phase after 12 hours. The output of L-lactic acid was 18.2g / L when cultured for 20h.
Embodiment 3
[0027] Embodiment 3, Lactobacillus salivarius XJP2 is to the tolerance determination of acid, bile salt
[0028] The activated XJP2 bacterial solution was mixed with 1% (about 10 7 cfu / mL) were inoculated in MRS medium with pH of 2.5, 3.5, and 4.5, respectively, and cultured statically at 37°C. Samples were taken at 0h, 2h, and 4h, and MRS was poured and counted. Treat the bacterial strain in the same way, inoculate the bacterial liquid in the MRS medium with pig bile salt concentration of 0.1%, 0.2%, and 0.3%, and culture it statically at 37°C. Samples were taken at 0h, 2h, and 4h, and MRS was poured and counted.
[0029] From the results in Table 1 and Table 2, it can be seen that the strain XJP2 has good tolerance to acid and bile salts, and the survival rate of viable bacteria exceeds 70% after being cultured in MRS medium with pH 2.5 or 0.3% bile salts for 2 hours , has better stress resistance.
[0030] Table 1 The results of acid tolerance of strain XJP2 (cfu / mL)
...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap