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Preparation method and application of electrochemiluminescence immunosensor based on Luminol/Au@branched PPy

A luminescence immunology and electrochemistry technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, scientific instruments, etc., can solve the limitation of the development of clinical detection technology for early detection of tumor markers, long operation time , radioactive pollution and other problems, to achieve the effect of enhancing electrochemiluminescence signal, high sensitivity and high sensitivity

Active Publication Date: 2016-06-01
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the development of biological technology and the deepening of research, it has been found that one of the most notable characteristics of many early tumor markers is their very low concentration in serum, while traditional methods for detecting tumor markers, such as enzyme-linked immunoassay , radioimmunoassay and fluorescence immunoassay, etc., have the disadvantages of radioactive pollution, long operation time and low sensitivity, which limit the development of clinical detection technology for early detection of tumor markers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation method of electrochemiluminescence immunosensor based on Luminol / AubranchedPPy

[0030] (1) Polish the glassy carbon electrode with a diameter of 4mm with 1.0, 0.3, 0.05μm alumina powder in sequence, clean it with ultrapure water, and dry it with nitrogen;

[0031] (2) Drop 6μL of antibody capture substrate material Luminol-AubranchedPPy-Ab solution with a concentration of 1mg / mL on the electrode surface, 4 ° C save;

[0032] (3) Block non-specific active sites with 3 μL of 0.5% bovine serum albumin solution, ° C incubate in the refrigerator for 1 hour and clean;

[0033] (4) Drop 6 μL of a series of tumor marker antigens with a concentration of 0.01pg / mL~10ng / mL on the surface of the electrode, incubate for 1h at room temperature, clean it, ° C stored in the refrigerator for later use.

Embodiment 2

[0034] Example 2 A method for preparing an electrochemiluminescence immunosensor based on Luminol / AubranchedPPy, which is characterized in that it comprises the following steps:

[0035] (1) Polish the glassy carbon electrode with a diameter of 4mm with 1.0, 0.3, 0.05μm alumina powder in sequence, clean it with ultrapure water, and dry it with nitrogen;

[0036] (2) Drop 6μL of antibody capture substrate material Luminol-AubranchedPPy-Ab solution with a concentration of 2mg / mL on the electrode surface, 4 ° C save;

[0037] (3) Use 3μL of bovine serum albumin solution with a mass fraction of 1.0% to block non-specific active sites. ° Incubate in refrigerator C for 2 hours and clean;

[0038] (4) Drop 6 μL of a series of tumor marker antigens with a concentration of 0.01pg / mL~10ng / mL on the surface of the electrode, incubate for 2h at room temperature, clean it, ° C stored in the refrigerator for later use.

Embodiment 3

[0039] Example 3 Preparation method of electrochemiluminescence immunosensor based on Luminol / AubranchedPPy

[0040] (1) Polish the glassy carbon electrode with a diameter of 4mm with 1.0, 0.3, 0.05μm alumina powder in sequence, clean it with ultrapure water, and dry it with nitrogen;

[0041] (2) Drop 6μL of antibody capture substrate material Luminol-AubranchedPPy-Ab solution with a concentration of 3mg / mL on the electrode surface, 4 ° C save;

[0042] (3) Use 3μL of bovine serum albumin solution with a mass fraction of 1.5% to block non-specific active sites. ° Incubate in refrigerator C for 3 hours and clean;

[0043] (4) Drop 6 μL of a series of tumor marker antigens with a concentration of 0.01pg / mL~10ng / mL on the surface of the electrode, incubate for 3h at room temperature, clean it, ° C stored in the refrigerator for later use.

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Abstract

The invention relates to a preparation method and application of an electrochemiluminescence immunosensor based on Luminol / Au@branched PPy, and belongs to the field of an electrochemiluminescence sensor. Particularly, nanometer composite materials Au@branched PPy are used; Luminol is used as an electrochemiluminescence signal source, and the electrochemiluminescence immunosensor for detecting tumor markers is prepared. A single-order circulation pulse method is used for realizing the detection on the electrochemiluminescence intensity of objects to be detected at different concentrations.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemiluminescent immunosensor based on Luminol / AubranchedPPy. It specifically relates to a Luminol / AubranchedPPy as a base material and Luminol as a luminescence signal source to construct a label-free electrochemiluminescence immunosensor, which belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Cancer is one of the most serious diseases that threaten human health. Although the treatment technology of malignant tumors is constantly improving, so far, early detection, early diagnosis and early treatment of malignant tumors are the most effective means. From a screening point of view, the most effective method for early diagnosis of cancer is through blood tests to detect the content of tumor markers. With the development of biological technology and the deepening of research, it has been found that one of the most notable feature...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403G01N21/76
CPCG01N21/76G01N27/403G01N2800/00
Inventor 朱文娟魏琴吴丹范大伟孙旭胡丽华马洪敏张勇庞雪辉杜斌
Owner UNIV OF JINAN
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