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Preparation method and application of UTY antibody nano-particles for fast separation of X/Y sperms of cattle and sheep

A nanoparticle and sperm technology, applied in biochemical equipment and methods, viruses/phages, chemical instruments and methods, etc., can solve the problems of semen mechanical damage, expensive equipment and other problems, achieve low molecular weight, low immunogenicity, small damage effect

Inactive Publication Date: 2016-06-08
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Separation of X / Y sperm by flow cytometry requires expensive equipment and professional operators, and the semen will be damaged by ultraviolet radiation and mechanical damage during pre-treatment

Method used

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  • Preparation method and application of UTY antibody nano-particles for fast separation of X/Y sperms of cattle and sheep
  • Preparation method and application of UTY antibody nano-particles for fast separation of X/Y sperms of cattle and sheep
  • Preparation method and application of UTY antibody nano-particles for fast separation of X/Y sperms of cattle and sheep

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Embodiment Construction

[0025] The present invention is described in detail below in conjunction with embodiment.

[0026] After rigorous comparison, the applicant found that the antigenic peptide UTY has a high homology in the male-derived cells of sheep, goats and cattle, and then used its encoding gene as a candidate gene for the H-Y antigen. The candidate gene is located in the non-recombination region of the Y chromosome and is only in Expressed in male tissues. Therefore, the experiment established a nanoparticle platform for encapsulating monoclonal antibodies, and isolated the antigen-binding fragment Fab' of the monoclonal antibody (anti-UTY) with high specificity for the UTY antigen highly expressed in Y sperm, and then combined it with The nanoparticles are cross-linked to form UTY antigen-specific monoclonal antibody nanoparticles.

[0027] 1. Preparation of UTY antibody nanoparticles

[0028] 1. Cloning UTY gene

[0029] Because the UTY gene sequence is highly conserved among cattle, ...

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Abstract

The invention discloses a preparation method and an application of UTY antibody nano-particles for fast separation of X / Y sperms of cattle and sheep. The preparation method comprises steps as follows: a monoclonal antibody resisting a Y sperm UTY antigen is prepared from Y sperm UTY antigen recombinant protein; the monoclonal antibody is subjected to enzyme digestion by pepsase, and Fab' fragments resisting an anti-UTY antigen are obtained through separation; calcium phosphate nano-particles containing the Fab' fragments are prepared. The prepared UTY antibody nano-particles have the capacity of performing antigen-antibody reaction on the surfaces of the Y sperms and inside the Y sperms, thereby leading to Y sperm agglutination, the separation efficiency of X / Y sperms reaches 80%-95%, and the UTY antibody nano-particles have the characteristics of simple, fast and cheap X / Y sperm separation, high efficiency and the like and has extremely small damage.

Description

technical field [0001] The invention relates to the field of rapid breeding of livestock, in particular to a preparation method and application of UTY antibody nanoparticles used for rapid separation of cattle and sheep X / Y sperm. Background technique [0002] Once the sex control (sex control) of domestic animals can be accurately grasped by humans, the economic benefits of animal husbandry will increase exponentially. Under normal circumstances, mammals are fertilized by the sperm carrying the Y chromosome (Y sperm) and the egg, and the fertilized egg develops into a male; while the sperm carrying the X chromosome (X sperm) combines with the egg, and the fertilized egg develops into a male. female development. Therefore, the direction of sexual development depends on the type of sperm involved in fertilization, and the purpose of controlling the sex of domestic animals can be achieved by artificially selecting a certain type of sperm to combine with eggs for fertilization...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N15/70C12N5/076
CPCC07K16/18C07K2317/55C12N5/061C12N15/70C12N2800/101
Inventor 华松张涌付明哲
Owner NORTHWEST A & F UNIV
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