Rice anther high-expression promoter OsAnth4 and application thereof

A promoter and anther technology, applied in the fields of biotechnology and crop genetic engineering, can solve problems such as gene silencing, co-suppression, unfavorable plant growth, etc.

Active Publication Date: 2016-06-08
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the promoters used in plant genetic engineering are constitutive promoters, which enable high-level expression of exogenous target genes in various plant tissues. Space effectively regulates the expression of the target gene, excessively consumes the material and energy in

Method used

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  • Rice anther high-expression promoter OsAnth4 and application thereof
  • Rice anther high-expression promoter OsAnth4 and application thereof
  • Rice anther high-expression promoter OsAnth4 and application thereof

Examples

Experimental program
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Embodiment 2

[0064] In order to verify that the sequence in SEQIDNo. ​​2 of the sequence list of the present invention also has a promoter function, the present invention uses other primers for this sequence to conduct an experiment similar to that in Example 1, namely: using rice variety Nipponbare DNA as a template and using New forward primers and reverse primers are used for promoter amplification; the target fragments amplified by PCR are recovered, connected to the PGEM-T-Easy vector, and transferred to activated competent cells; then, use T4 with the pCAMBIA1391 fragment The ligase is connected to obtain the corresponding crop expression vector, and the crop expression vector is transferred into Agrobacterium tumefaciens by freeze-thaw method; Agrobacterium-mediated rice genetic transformation is carried out on mature rice seeds to cultivate regenerated plants; finally, dyeing verification is performed.

[0065] Experiments have shown that the sequence in SEQIDNo.2 in the sequence listi...

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Abstract

The invention provides a rice anther high-expression promoter OsAnth4 and application thereof. The invention further provides an expression cassette containing the promoter and a recombinant expression vector. The inventor discovers, extracts and identifies a DNA sequence, with transcriptional control activity, in Nipponbare. The promoter is applied to crop gene engineering and used for culturing transgenic plants. The promoter can specifically drive target genes to perform high expression in anther, and accordingly the promoter can be effectively applied to rice fertility improvement and applied to hybrid rice breeding. The rice anther high-expression promoter is connected into a vector to have the gene upstream region capable of improving rice anther character and function, and accordingly the recombinant expression vector is built; the recombinant expression vector is transferred into rice cells, tissue or organs for culturing to obtain corresponding transgenic plants; in the plants, the promoter can drive the target genes to perform specific expression in the anther so as to improve the rice fertility.

Description

Technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice anther strong expression promoter and its application. The promoter can drive the strong expression of target genes in anthers in crop genetic engineering, thereby improving crop fertility. Background technique [0002] As an important regulatory element at the transcription level, the promoter regulates gene expression in different developmental stages and tissues. Generally, there are several elements that control tissue-specific expression at the same time in the promoter, and the expression specificity is determined by the type, number and relative position of these elements. In-depth study of these promoters will not only help clarify the basic theories of plant morphology, development, and metabolic pathways, but also have a wide range of applications. [0003] According to their transcription mode and func...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/84C12N1/21A01H5/00
CPCC12N15/113C12N15/8231C12N2310/10
Inventor 杨亚春杨剑波李浩魏鹏程李娟李莉秦瑞英许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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