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A device and method for measuring the adsorption capacity of soil for amino acids

An adsorption capacity, amino acid technology, applied in the field of plant nutrition and soil scientific research, can solve the problems of difficult to reflect the real state of soil, difficult to ensure the accuracy of research, changes in soil physical and chemical properties, etc., to achieve good sterilization effect and high accuracy , Reasonable effect of device design

Inactive Publication Date: 2017-09-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil sterilization is an important method to solve such problems. However, the physical and chemical properties of soil after autoclaving have undergone major changes, and it is difficult to reflect the true state of the soil.
Even after being sterilized by autoclaving, the follow-up series of studies are easily infected with microorganisms, and it is difficult to guarantee the accuracy of the research

Method used

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  • A device and method for measuring the adsorption capacity of soil for amino acids
  • A device and method for measuring the adsorption capacity of soil for amino acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] see figure 1 , a device for measuring the adsorption capacity of amino acids by soil, comprising a syringe 1, a first valve 2, a first filter 3, a soil column 4, a steel wire support net 5, a second valve 6, a second filter 7, a fourth Valve 8, the third filter 9, vacuum pump 10, the fifth valve 11, air tank 12, beaker 13, the sixth valve 14, the seventh valve 15, liquid collection bottle 16, the third valve 17; vacuum pump 10, storage The air tank 12 and the soil column 4 are connected in sequence, the injector 1, the first valve 2, and the first filter 3 are located on the upper part of the soil column 4, and the injector 1, the first valve 2, and the first filter 3 are connected in sequence, and the second The valve 6 and the second filter 7 are located on one side below the soil column 4, the steel wire support net 5 is located at the lower part of the soil column 4, the seventh valve 15 is arranged at the lower part of the soil column 4, and the liquid collection b...

Embodiment 2

[0028] (1) Device preparation: Air-dry the wetland soil in the west of Zijingang and pass it through a 2mm sieve. Take 50g of the sieved soil and add it to the soil column 4. After adding 5ml of chloroform to the beaker 13, place it in the air storage tank 12. Then press the above device to figure 1 connect them;

[0029] (2) Preparation of chloroform gas: close the first valve 2, the second valve 6, the fifth valve 11, the seventh valve 15, open the fourth valve 8, the sixth valve 14, the third valve 17, open the vacuum pump 10, After vacuuming, first close the third valve 17, then close the vacuum pump 10, and then close the fourth valve 8. After the chloroform in the beaker 13 is completely converted into gas, open the second valve 6 and put a small amount of air to maintain the internal pressure of the device. Same as the outside world, immediately close the second valve 6, open the third valve 17, the fifth valve 11, and keep the fourth valve 8 and the sixth valve 14 in ...

Embodiment 3

[0035] (1) Device preparation: After naturally air-drying the wetland soil in the west of Zijingang, pass it through a 2mm sieve, take 50g of the sieved soil and add it to the soil column 4, add 5ml of chloroform to the beaker 13, and place it in the air storage tank 12, Then press the above device to figure 1 connect them;

[0036] (2) Preparation of chloroform gas: close the first valve 2, the second valve 6, the fifth valve 11, the seventh valve 15, open the fourth valve 8, the sixth valve 14, the third valve 17, open the vacuum pump 10, After vacuuming, first close the third valve 17, then close the vacuum pump 10, and then close the fourth valve 8. After the chloroform in the beaker 13 is completely converted into gas, open the second valve 6 and put a small amount of air to maintain the internal pressure of the device. Same as the outside world, immediately close the second valve 6, open the third valve 17, the fifth valve 11, and keep the fourth valve 8 and the sixth v...

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Abstract

The invention provides a device for determining amino acid adsorption capacity of soil. The device comprises an injector, a first valve, a first filter, a soil mass column, a steel wire supporting net, a second valve, a second filter, a fourth valve, a third filter, a vacuum pump, a fifth valve, a gas holder, a beaker, a sixth valve, a seventh valve, a liquid collection bottle and a third valve. Microorganisms in the soil and the device are killed by trichloromethane, influence of residues is avoided effectively, and an isotope labeled amino acid solution with high concentration is adopted to study the adsorption characteristic of the soil for certain amino acid. The device has the advantages of reasonable design, simplicity in operation, good sterilizing effect, high soil determining accuracy and capability of effective determination of the amino acid adsorption capacity of the soil.

Description

technical field [0001] The invention belongs to the field of plant nutrition and soil science research, and relates to a device and method for measuring the adsorption capacity of soil to amino acids. Background technique [0002] The research results of the past two decades have shown that plants have the ability to absorb molecular amino acids without the need for microorganisms to decompose amino acids into inorganic nitrogen and then be absorbed and utilized by plants. The soil contains a variety of amino acids with high concentrations, and the organic matter in the soil can continuously produce a large amount of amino acids under the action of microorganisms. In addition, compared with inorganic nitrogen such as ammonium nitrogen and nitrate nitrogen, amino acids play an important role in improving crop quality, and the pollution to the environment is relatively small. Therefore, in-depth study of the physical and chemical processes such as the composition, concentrati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/72G01N30/06
CPCG01N30/06G01N30/72
Inventor 马庆旭吴良欢
Owner ZHEJIANG UNIV
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