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Amauroderma rugosum sporocarp culture method

A cultivation method and technology of Uzhi seedlings, applied in the cultivation field of Uzhi fruiting bodies, can solve problems such as not being able to obtain fruiting bodies of Uzhi zhi, lack of commercial utilization, and undeveloped utilization, etc., and achieve coarse fruiting bodies The effect of high polysaccharide content, shortening the seed production time and saving seed production

Active Publication Date: 2016-06-15
韶关市星河生物科技有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are more than 25 kinds of wild ganoderma growth records in the world, but less than 10% of them have been commercially utilized, and most of the wild ganoderma resources have not been developed and utilized.
However, there is currently no relevant literature report on the cultivation of fruiting bodies of Uchida chinensis
[0005] The growth characteristics of the fruiting body of Wuzhi (also known as false lucidum) are different from those of conventional varieties such as red Ganoderma and purple Ganoderma currently cultivated, and the conventional cultivation and management of Ganoderma cannot be used to obtain fruiting bodies of Wuzhi.

Method used

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  • Amauroderma rugosum sporocarp culture method
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  • Amauroderma rugosum sporocarp culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method for cultivating Wuzhi, the method steps are as follows:

[0038] (1), pick part of the pure mycelium of Wuzhi (0.5cm 2 ) inoculated into a new test tube culture medium (medium formula: 20% peeled potato, 2% glucose, 0.2% peptone, 0.3% potassium dihydrogen phosphate, 2% agar, 0.15% magnesium sulfate, VB 1 0.003%, pH7-8), cultured for 10-15 days under certain conditions (darkness, ambient temperature 25±1°C, relative air humidity 60%-75% and other suitable conditions) to obtain the strains available for reproduction—Class I species (mother species).

[0039] (2) Use a sterilized inoculation hook to pick up the parent seed of the test tube 0.5cm 2 10 seed pieces, inoculated into a 250mL Erlenmeyer flask containing 100mL liquid medium (medium formula: 20% peeled potato, 2% glucose, 0.2% peptone, 0.2% beef extract, 0.3% potassium dihydrogen phosphate, sulfuric acid Magnesium 0.15%, VB 1 0.003%, pH7-8), placed on a rotary shaker, 26±1°C, 120-150rpm shaking cultur...

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Abstract

The invention discloses an amauroderma rugosum sporocarp culture method.The method includes the following steps of obtaining an amauroderma rugosum liquid strain through a mother strain, a shake flask strain and a fermentation strain; inoculating the amauroderma rugosum liquid strain to amauroderma rugosum culture medium fungus bags, and obtaining amauroderma rugosum production fungus bags after fungi grow on the whole fungus bags; conducting after-ripening for 7-15 days after fungi grow on the whole fungus bags, and then transferring the fungus bags out of an amauroderma rugosum house to conduct shelf type basket-carrying amauroderma rugosum output management, wherein culture baskets applied to amauroderma rugosum output management are not lower than fungus bags by 15 cm, the space between every two adjacent fungus bags is 10-20 cm wide, and the tops of the culture baskets and the parts, higher than the fungus bags, of the culture baskets are wrapped and sealed by films so that amauroderma rugosum culture can be achieved; continuing to culture harvestable amauroderma rugosum sporocarp after sporocarp primordium is formed.According to the growth and development characteristics and research tests of amauroderma rugosum sporocarp, the scheme for artificially culturing amauroderma rugosum sporocarp is found.A new species is added to the species of edible fungi artificially cultured, and a firm foundation is laid for research and commercial development of amauroderma rugosum.

Description

technical field [0001] The invention specifically relates to a method for cultivating fruiting bodies of Uchid chinensis. Background technique [0002] With the development of human society, people's voice of "advocating nature and returning to nature" is getting higher and higher, and people's social demand for natural medicine and natural health care products is increasing day by day. Edible and medicinal fungi are favored for their multi-faceted biological activities, safety and non-toxic side effects. Excavating new edible and medicinal fungi resources and carrying out artificial domestication and cultivation have become a research hotspot in the edible and medicinal fungi industry. As the "king of medicinal fungi", Ganoderma lucidum has long been recognized by the world for its health care and medicinal effects. "Ganoderma lucidum" is actually a general term for different species of Ganoderma lucidum in the family Ganodermaceae. In a broad sense, Ganoderma lucidum incl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
CPCA01G18/00C12N1/14
Inventor 肖自添何焕清刘明
Owner 韶关市星河生物科技有限公司
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