Amauroderma rugosum sporocarp culture method
A cultivation method and technology of Uzhi seedlings, applied in the cultivation field of Uzhi fruiting bodies, can solve problems such as not being able to obtain fruiting bodies of Uzhi zhi, lack of commercial utilization, and undeveloped utilization, etc., and achieve coarse fruiting bodies The effect of high polysaccharide content, shortening the seed production time and saving seed production
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[0037] A method for cultivating Wuzhi, the method steps are as follows:
[0038] (1), pick part of the pure mycelium of Wuzhi (0.5cm 2 ) inoculated into a new test tube culture medium (medium formula: 20% peeled potato, 2% glucose, 0.2% peptone, 0.3% potassium dihydrogen phosphate, 2% agar, 0.15% magnesium sulfate, VB 1 0.003%, pH7-8), cultured for 10-15 days under certain conditions (darkness, ambient temperature 25±1°C, relative air humidity 60%-75% and other suitable conditions) to obtain the strains available for reproduction—Class I species (mother species).
[0039] (2) Use a sterilized inoculation hook to pick up the parent seed of the test tube 0.5cm 2 10 seed pieces, inoculated into a 250mL Erlenmeyer flask containing 100mL liquid medium (medium formula: 20% peeled potato, 2% glucose, 0.2% peptone, 0.2% beef extract, 0.3% potassium dihydrogen phosphate, sulfuric acid Magnesium 0.15%, VB 1 0.003%, pH7-8), placed on a rotary shaker, 26±1°C, 120-150rpm shaking cultur...
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