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Method for cultivating passion fruit virus-free seedlings

A cultivation method and technology of passion fruit, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of carrying and ineffective planting, and achieve the effects of simple operation, reduced disease rate, and improved economic benefits

Inactive Publication Date: 2016-06-29
龙岩市禾康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of cultivation method of passion fruit virus-free seedlings, obtain passion fruit virus-free seedlings through 5 steps such as pretreatment, basic culture, virus-free culture, multiplication tissue culture rapid propagation culture, rooting culture, effectively solve The difficult problem that passion fruit tissue culture seedlings carry virus and can't be planted effectively, compared with conventional non-virus-free passion fruit seedlings, the incidence of disease after planting of passion fruit virus-free seedlings of the present invention is only 2%, improves the economic benefit of passion fruit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for cultivating passion fruit virus-free seedlings, cultivated according to 5 steps of plant explant pretreatment, basic culture, virus-free culture, proliferation tissue culture rapid propagation culture and rooting culture, and the steps are as follows:

[0026] (1) Pre-treatment of plant explants:

[0027] Select the stem tips of healthy passion fruit plants, wash the surface of the stem tips of the plants with sterile water for 3 times, soak the stem tips of the plants in disinfectant for 20 minutes, remove some microorganisms on the surface of the stem tips of the plants, and then place the stem tips of the plants at 45 After soaking in water at ℃ for 45 minutes, the stem tip of the plant was again soaked in disinfectant for 15 minutes;

[0028] The ratio of each component in the described disinfectant is: by mass percentage, antibiotic 0.004%, hymexazol 0.015%, methexene 0.04%, propamocarb hydrochloride 0.08%, cresol sulfonic acid 0.02% , the balance is ...

Embodiment 2

[0044] A method for cultivating passion fruit virus-free seedlings, cultivated according to 5 steps of plant explant pretreatment, basic culture, virus-free culture, proliferation tissue culture rapid propagation culture and rooting culture, and the steps are as follows:

[0045] (1) Pre-treatment of plant explants:

[0046] Select the stem tips of healthy passion fruit plants, wash the surface of the stem tips of the plants with sterile water 4 times, soak the stem tips of the plants in disinfectant for 18 minutes, remove some microorganisms on the surface of the stem tips of the plants, and then place the stem tips of the plants at 43 After soaking in water at ℃ for 53 minutes, the stem tip of the plant was again soaked in disinfectant for 12 minutes;

[0047] The proportion of each component in the described disinfectant is: by mass percentage, antibiotic 0.003%, hymexazol 0.010%, urotropine 0.03%, propamocarb hydrochloride 0.07%, cresol sulfonic acid 0.015% %, the balance...

Embodiment 3

[0063] A kind of cultivation method of passion fruit virus-free seedling, cultivates according to 5 steps of plant explant pretreatment, basic culture, virus-free culture, multiplication tissue culture rapid propagation culture, rooting culture, and described step is specifically as follows:

[0064] (1) Pre-treatment of plant explants:

[0065] Select the stem tips of healthy passion fruit plants, wash the surface of the stem tips of the plants with sterile water 5 times, soak the stem tips of the plants in disinfectant for 15 minutes, remove some microorganisms on the surface of the stem tips of the plants, and then place the stem tips of the plants at 42 After soaking in water at ℃ for 60 minutes, the stem tip of the plant was again soaked in disinfectant for 10 minutes;

[0066] The ratio of each component in the described disinfectant is: by mass percentage, antibiotic 0.002%, hymexazol 0.005%, hexamethamine 0.02%, propamocarb hydrochloride 0.06%, cresol sulfonic acid 0.0...

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Abstract

The invention discloses a method for cultivating passion fruit virus-free seedlings. The passion fruit virus-free seedlings are obtained by five steps of plant explanting preprocessing, basic cultivating, detoxication cultivating, multiplication tissue cultivation rapid propagation cultivating and rooting cultivating. The method for cultivating the passion fruit virus-free seedlings is simple in operation, and can be used for industrially producing a large batch of passion fruit virus-free seedlings; after the virus-free seedlings are planted, the disease rate of passion fruit plants is effectively reduced; compared with common non-detoxification passion fruit seedlings, the passion fruit virus-free seedlings have the advantage that the disease rate is only 2% after being planted, and the economic benefits of passion fruits are greatly increased.

Description

technical field [0001] The invention belongs to the field of cultivating virus-free seedlings of plants, and in particular relates to a method for cultivating virus-free seedlings of passion fruit. Background technique [0002] passion fruit( Passiflora edulis Sims), scientific name Passiflora, Passiflora, Passiflora, is a tropical, subtropical perennial evergreen vine berry fruit tree. It is named passion fruit because its juice exudes the aroma of more than a hundred kinds of fruits such as peach, strawberry, lemon, mango, pineapple, banana, pomegranate, and sour plum. Passion fruit planting diseases and insect pests are serious at present, that is, the roots of passion fruit are rotted after planting; the outbreak of diseases and insect pests of surviving passion fruit plants is serious, resulting in the use of pesticides for prevention and control in large quantities; the fruits of passion fruit plants are seriously shedding, and the yield is reduced. The seedlings of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 樊仲书王海斌张玉华樊昌华孔祥海樊君早陈晓婷
Owner 龙岩市禾康生物科技有限公司
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