A micro-cutting rapid breeding method for castanopsis hystrix seedlings

A technology of micro-cutting and red cone, applied in the field of agricultural biology, can solve the problems of low callus differentiation, long time, slow elongation of adventitious buds, etc. Effect

Inactive Publication Date: 2016-07-06
张秀国
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Problems solved by technology

[0004] In recent years, cutting propagation and tissue culture propagation have become the research focus of red cone seedling propagation, and the survival rate of cutting propagation is low; tissue culture propagation currently adopts methods of callus induction, callus differentiation and proliferation, and adventitious bud rooting to obtain sterile seedlings , the problems in this way

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Embodiment Construction

[0015] The present invention will be further described below.

[0016] A method for rapidly propagating red cone seedlings by micro-cutting provided by the present invention comprises the process of obtaining sterile seedlings, the process of micro-cutting of stem segments, and the process of rooting stem segments.

[0017] 1) The process of obtaining sterile vaccines

[0018] Select the nuts of red cones after disinfection, peel off the seeds, inoculate the seeds into the germination medium and cultivate them until they grow into seedlings. The cultivation temperature is 25-28°C, and the light intensity is 15-20 μmol·m -2 ·s -1 , the light time is 10h·d -1 , cultivated for 30 to 40 days; the germination medium is based on MS medium and supplemented with 0.2 mg·L of 6-benzyl adenine -1 , α-Naphthylacetic acid 0.05mg·L -1 .

[0019] 2), the micro-cutting process of stem segments

[0020] Cut the seedlings obtained in step 1 into stem segments with 1-2 nodes, insert the ba...

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Abstract

A micro-cutting rapid breeding method for castanopsis hystrix seedlings is disclosed. The method includes inoculating a castanopsis hystrix seed to a germination culture medium, culturing to obtain a seedling, cutting the seedling into stem segments, inserting the stem segments into a micro-cutting culture medium, culturing until lateral buds occur, cutting the lateral buds into stem segments, inserting the stem segments into a fresh micro-cutting culture medium, culturing, cutting the obtained lateral buds into stem segments, inserting the stem segments into a rooting culture medium, and culturing until the stem segments root, thus preparing the castanopsis hystrix seedlings. The method is simple and feasible in process and high in breeding speed, overcomes the problem that castanopsis hystrix seeds are difficult to collect due to biennial bearing, achieves long-term large-scale rapid breeding of the castanopsis hystrix seedlings, can prepare high-quality castanopsis hystrix test-tube plantlets in a large-scale, rapid and continuous manner, and is high in practicality.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to a method for rapidly propagating red cone seedlings by micro-cutting of stem segments. Background technique [0002] Red cone (Castanopsishystrix) is an evergreen tree of the Fagaceae Castanopsis genus. It is distributed in the southern parts of Guangxi, Guangdong, Hainan, Yunnan, Guizhou, Hunan, Jiangxi, Fujian and other provinces, as well as Medog County in Tibet. The central distribution area is in the southern part of Guangxi Hilly areas below 700m above sea level. It grows fast, has strong germination, dense branches and leaves, is more shade-tolerant, and has good mixed growth performance. It is the best tree species for mixed afforestation with pine, fir or other broad-leaved trees in the hilly areas of South China. Red cone material is excellent, the wood is hard and corrosion-resistant, the heartwood is large, brownish red, and the sapwood is light r...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张秀国孙仁杰魏秀芝
Owner 张秀国
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