Vaccine for expressing panda canine distemper virus H and F gene recombinant goat pox virus, and preparation method and immuno application method thereof

Abstract

The invention discloses a vaccine for expressing panda canine distemper virus H and F gene recombinant goat pox virus.The vaccine comprises recombinant goat pox virus vpTK-H-Eg or vpTK-F-Eg obtained by using goat pox virus as a vector to transfect and express respectively recombinant transfer vector plasma pTK-H-Eg or pTK-F-Eg of panda canine distemper virus H or F gene, wherein the recombinant goat pox virus vpTK-H-Eg comprises amino acid sequences shown as in SEQ ID NO.1, and the recombinant goat pox virus vpTK-F-Eg comprises amino acid sequences shown as in SEQ ID NO.2.The vaccine developed herein has the advantages of safety, high efficiency, low production cost and the like, and basis is laid for the prevention and treatment of canine distemper.

Description

technical field [0001] The invention relates to the technical field of recombinant viruses, in particular to the technical field of construction of recombinant goat pox virus expressing H and F genes of giant panda canine distemper virus. Background technique [0002] Canine distemper (Canine Distemper, CD) is caused by canine distemper virus (Canine Distemper Virus, CDV) and is an acute, highly contagious infectious disease. CDV is a negative-sense, single-strand non-segmented RNA that belongs to the family Paramyxoviridae and the genus Morbillivirus. It often causes diseases in many animals such as Canidae, Weaselidae, Procyonidae and Felidae, and the incidence rate can reach almost 100%. Its clinical symptoms are diverse, and it is very easy to have secondary bacterial and viral infections or secondary infections, causing heavy economic losses and a mortality rate of up to 80%. It is known as a "devastating infectious disease". In recent years, the host range of CD has c...

AI Technical Summary

Problems solved by technology

Although inactivated vaccines and attenuated vaccines have a certain effect on the control of canine distemper, but with the widespread use, there are also many shortcomings, such as low immune effect of inactivated vaccines and low antibody levels; MV vaccines cannot provide lasting immunity. At present, atte

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