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The flanking sequence of the exogenous insert in transgenic rice Jishengjing 3

A transgenic rice and flanking sequence technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problem of flanking sequences of foreign inserts in the transgenic rice Jishengjing 3. Articles and patents Reporting and other issues

Active Publication Date: 2018-08-17
JILIN ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, some patents and documents have reported the flanking sequence of the exogenous insert in transgenic plants, but there are no articles or patent reports on the flanking sequence of the exogenous insert in the transgenic rice Jishengjing 3

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  • The flanking sequence of the exogenous insert in transgenic rice Jishengjing 3
  • The flanking sequence of the exogenous insert in transgenic rice Jishengjing 3
  • The flanking sequence of the exogenous insert in transgenic rice Jishengjing 3

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Embodiment Construction

[0013] The flanking sequence of the exogenous insert fragment of the transgenic rice Jishengjing 3, the flanking sequence is the flanking sequence at the 3' end, and the flanking sequence at the 3' end is shown in SEQ ID NO.7.

[0014] The method for preparing the flanking sequence of the exogenous insert in the transgenic rice Jishengjing 3 comprises extracting the genomic DNA of the transgenic rice Jishengjing 3 and separating it by genome walking and thermal asymmetric PCR.

[0015] The application of the flanking sequence of the exogenous insert fragment of transgenic rice Jishengjing 3 in the line-specific PCR detection method of Jishengjing 3.

[0016] The present invention will be further described below through specific experimental examples.

[0017] 1. Experimental materials

[0018] 1.1 Plant material

[0019] Transgenic insect-resistant rice line Jishengjing 3, non-transgenic rice Jishengjing 88.

[0020] 1.2 Enzymes and Reagents

[0021] Molecular biology reag...

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Abstract

The invention provides a flanking sequence of an exogenous insertion fragment of transgenic rice Jishengjing 3, belongs to the field of biotechnology, and is used for specific PCR detection of Jishengjing 3 strain. The flanking sequence is the flanking sequence at the 3' end, and the sequence is shown in SEQ ID NO.7, which provides nested primer sequences at the 3' and 5' ends of the T-DNA, using the total DNA of the rice to be tested as a template, through the genome step transfer method and thermal asymmetric PCR method, use the primers to carry out PCR detection, and isolate the 3' flanking sequence of the T-DNA sequence. The flanking sequence of the foreign source insert fragment isolated by the present invention can be further used to establish Jishengjing No. 3 Strain-specific PCR detection method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a flanking sequence of an exogenous insert fragment of a transgenic rice line Jishengjing No. 3, which is used in a line-specific PCR detection method of Jishengjing No. 3. Background technique [0002] Rice is one of the most important food crops in my country and even in the world. At present, global transgenic rice research is progressing rapidly, involving many aspects such as insect resistance, disease resistance, herbicide resistance, quality and agronomic traits improvement. The research and development of genetically modified rice in my country is in step with the world, and insect-resistant genetically modified rice is at the leading level in the world. Strain-specific detection of transgenic plants is an important technical basis for the supervision and management of transgenic plants to ensure their healthy development. The flanking sequence of the exogenous insert fragmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/6895
CPCC12N15/102C12Q1/6895C12Q2531/107
Inventor 林秀峰金永梅
Owner JILIN ACAD OF AGRI SCI