Endosperm-specific down-regulation of osmads7 gene expression to improve the tolerance of rice amylose content to high temperature

Abstract

The invention discloses a method for specifically down-regulating the expression of OsMADS7 gene in endosperm to improve the tolerance of rice amylose content to high temperature. The specific interference fragment of OsMADS7 gene is cloned from rice genome, and the promoter of rice glutenin gene is used. The endosperm-specific interference vector was constructed, and the interference vector was transferred into the rice genome through the Agrobacterium tumefaciens mediated method, and the gene expression identification and phenotype analysis of the transgenic plants were carried out. The beneficial effects of the present invention are as follows: the present invention successfully obtains a high-temperature responsive transcription factor OsMADS7, the expression of this gene in wild-type rice varieties can be induced by high temperature, and the expression of OsMADS7 is specifically interfered with by the endosperm, we found that the transgenic rice lines Amylose content has stronger tolerance to high temperature without affecting other important agronomic traits of rice, thus providing an effective way for people to effectively maintain the quality of rice under high temperature and filling the technical gap in related fields.

Description

technical field

[0001] The invention belongs to the field of plant genetic engineering, and specifically relates to a method for specifically down-regulating the expression of the OsMADS7 gene in endosperm and improving the tolerance of rice amylose content to high temperature. Background technique

[0002] The production practice and research results show that the rice yield and quality will be seriously affected when rice is exposed to high ambient temperature during the grain filling stage. High temperature-sensitive varieties, especially some high-quality japonica rice varieties, under the influence of high temperature, the amylose content of rice will decrease significantly, and at the same time, the chalkiness rate will increase significantly. There will also be a significant decline in the sperm yield, thereby reducing rice yield. Identifying the functional genes related to the effect of high temperature on rice quality, and using genetic engineering technology to ch...

Examples

Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with accompanying drawing:

[0030] 1. Rice material

[0031] The wild-type materials of rice (Oryza sativa) were japonica varieties Zhonghua 11 (ZH11) and Nipponbare (NIP). The high-temperature greenhouse is set at 35°C during the day, 12 hours of light; at night, 28°C, and 12 hours of darkness. The room temperature control was set at 28°C during the day, 12 hours of light; at night, 22°C, and 12 hours of darkness. On the 9th day after flowering, that is, after 6 days of temperature treatment, samples were taken, quickly frozen with liquid nitrogen, and stored in a -80°C ultra-low temperature refrigerator, and then taken out when ready to use.

[0032] 2. Rice processing and quality determination

[0033] The mature rice seeds were thoroughly dried, and the amylose determination and gelatinization temperature analysis were carried out after shelling and embryo removal.

[0034] Because the chang...

technical field

[0001] The invention belongs to the field of plant genetic engineering, and specifically relates to a method for specifically down-regulating the expression of the OsMADS7 gene in endosperm and improving the tolerance of rice amylose content to high temperature. Background technique

[0002] The production practice and research results show that the rice yield and quality will be seriously affected when rice is exposed to high ambient temperature during the grain filling stage. High temperature-sensitive varieties, especially some high-quality japonica rice varieties, under the influence of high temperature, the amylose content of rice will decrease significantly, and at the same time, the chalkiness rate will increase significantly. There will also be a significant decline in the sperm yield, thereby reducing rice yield. Identifying the functional genes related to the effect of high temperature on rice quality, and using genetic engineering technology to ch...

Examples

Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with accompanying drawing:

[0030] 1. Rice material

[0031] The wild-type materials of rice (Oryza sativa) were japonica varieties Zhonghua 11 (ZH11) and Nipponbare (NIP). The high-temperature greenhouse is set at 35°C during the day, 12 hours of light; at night, 28°C, and 12 hours of darkness. The room temperature control was set at 28°C during the day, 12 hours of light; at night, 22°C, and 12 hours of darkness. On the 9th day after flowering, that is, after 6 days of temperature treatment, samples were taken, quickly frozen with liquid nitrogen, and stored in a -80°C ultra-low temperature refrigerator, and then taken out when ready to use.

[0032] 2. Rice processing and quality determination

[0033] The mature rice seeds were thoroughly dried, and the amylose determination and gelatinization temperature analysis were carried out after shelling and embryo removal.

[0034] Because the chang...

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