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Lymph node metastasis carcinoma diagnostic method based on immunomagnetic beads and kit

A technique for lymph node metastases and lymph node metastases, which is applied in the field of nanomaterials and medicine, can solve the problems of atypical cells that are difficult to distinguish, the false negative rate of diagnosis, the limited puncture specimens, and the high false positive rate, and achieve high sensitivity, improve sensitivity, and operate easy effect

Inactive Publication Date: 2016-07-20
WUHAN UNIV
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Problems solved by technology

From this result, although needle aspiration cytology is a reliable and highly accurate method for pathological diagnosis of lymph nodes, there are still factors such as relying on the subjective judgment of doctors, limited biopsy samples, and difficulty in distinguishing and diagnosing atypical cells. Causes problems such as false negative rate and false positive rate to be solved urgently

Method used

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  • Lymph node metastasis carcinoma diagnostic method based on immunomagnetic beads and kit
  • Lymph node metastasis carcinoma diagnostic method based on immunomagnetic beads and kit
  • Lymph node metastasis carcinoma diagnostic method based on immunomagnetic beads and kit

Examples

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Embodiment 1

[0030] Example 1 Rapid Diagnosis of Lymph Node Metastatic Cancer

[0031] Schematic diagram of using immunomagnetic beads (IMNs) to detect cancer cells in lymph node puncture cells. figure 1 As shown, the method of immunomagnetic sorting can specifically capture the metastatic cancer cells (epithelial origin) in the puncture, without the release of magnetic balls, and it is convenient for a series of subsequent observations and identifications, including Wright's staining. Observation of cancer cell morphology, immunocytochemical identification, immunohistochemical identification and molecular level identification of fluorescence in situ hybridization.

[0032] The method for establishing a breast cancer cell MCF-7 as a cell model and using magnetic nanometers to reliably diagnose lymph node metastases will be described in detail below.

[0033] 1. Method

[0034] 1. Preparation of Magnetic Nanospheres (MNs)

[0035] Magnetic nanospheres are assembled layer by layer on the ...

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Abstract

The invention discloses a lymph node metastasis carcinoma diagnostic method based on immunomagnetic beads and a kit. According to the method, the based immunomagnetic beads refer to magnetic nanospheres modified with anti-epithelial adhesion molecular antibodies. The method comprises the following steps: adding immunomagnetic beads into a paracentesis specimen for incubating for 5-10 minutes, performing magnetic separation, washing, smearing, staining and other steps, and realizing sensitive detection and visualization diagnosis of metastatic cancer cells. The method disclosed by the invention is high in capture efficiency and specificity and small in influence of cancer cell morphometry, and the total diagnosis accuracy rate is 99.1%. According to the method disclosed by the invention, distinctive diagnosis of lymphoid reactive hyperplasia, malignant lymphoma and metastatic carcinoma is realized, the cell smear subjected to magnetic separation can be directly used for identifications such as Wright staining, immunocytochemistry, immunohistochemistry and fluorescent in-situ hybridization, and the method becomes a powerful means for diagnosis of metastatic cancers and has important medical significances.

Description

technical field [0001] The invention belongs to the field of nanomaterials and medicine, and in particular relates to an immunomagnetic ball-based lymph node metastasis cancer diagnosis method and a kit. Background technique [0002] Magnetic and bio-targeting functional nanospheres have realized the detection of nucleic acids, viruses, bacteria, protein molecules and tumor cells, and have been widely reported and applied in the field of biomedicine. Xie et al. (Fluorescent-magneticdual-encoded nanospheres: apromising tool for fast-simultaneous-addressable high-throughputanalysis, Nanotechnology, 2012, 23(3), p. 035602) realized the magnetic, fluorescent and biological targeting of micro-nanospheres for the first time by layer-by-layer assembly method Triple coded. Wen et al. (Quick-Response Magnetic Nanospheres for Rapid, Efficient Capture and Sensitive Detection of Circulating Tumor Cells, Acs. Nano., 2014, 8 (1), pages 941-949) constructed a magnetic nanosphere with a si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02
CPCC12N5/0693C12N2501/58C12N2509/00G01N33/5017
Inventor 秦银辉庞代文张志凌吴玲玲夏和顺漆楚波
Owner WUHAN UNIV
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