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Method for improving survivability of old people mesenchymal stem cells after transplanting

A technology of bone marrow mesenchyme and viability, applied in bone/connective tissue cells, biochemical equipment and methods, cells modified by introducing foreign genetic material, etc., to achieve the effect of improving viability and improving effect

Active Publication Date: 2016-07-27
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

So far, although there are many ways to improve the survival rate of bone marrow-derived mesenchymal stem cells after transplantation, there is no report on the improvement of the survival rate and curative effect of bone marrow-derived mesenchymal stem cells derived from elderly individuals

Method used

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  • Method for improving survivability of old people mesenchymal stem cells after transplanting
  • Method for improving survivability of old people mesenchymal stem cells after transplanting
  • Method for improving survivability of old people mesenchymal stem cells after transplanting

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Embodiment 1

[0019] 1. Under aseptic conditions, extract 10ml of bone marrow fluid from suitable elderly patients (excluding hematopoietic system disorders) (Mei Moumou, 85 years old, from Hangzhou, I agree to donate bone marrow for research) hip replacement surgery, and place it in advance Add 4000IU heparin to a sterile glass bottle, mix thoroughly to avoid coagulation of bone marrow fluid, and move to the biological safety cabinet of the laminar flow room for the next step.

[0020] 2. Add an equal amount of low-sugar DMEM medium (add 100U / ml penicillin and 100U / ml streptomycin) (purchased from GIBCO, USA) into a sterile glass bottle containing bone marrow fluid to dilute the bone marrow fluid, and use a sterile pipette Fully blow out bone marrow cells.

[0021] 3. Separate bone marrow mononuclear cells according to density gradient separation method. Add 20ml of lymphocyte separation solution (density 1.077±0.001g / ml, purchased from Shanghai Hengxin Chemical Reagent Co., Ltd.) into a 50ml ...

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Abstract

The invention provides a method for improving the survivability of old people mesenchymal stem cells after transplanting. According to the method, mesenchymal stem cells with passage stability obtained from the bone marrow of old people are put into a conventional culture medium in which 0.5mumol / L SRT1720, 5mM NAD+ and 20mM glutamine are added; pretreatment is performed for 24 hours; the obtained and processed mesenchymal stem cells are used for cell transplanting treatment. The method has the beneficial effects that for the SRT1720 pretreatment, the apoptosis of the old people mesenchymal stem cells under the stress condition can be inhibited through inhibiting an exogenous apoptosis path of the old people mesenchymal stem cells; after the transplanting of the mesenchymal stem cells subjected to the SRT1720 pretreatment, the survival rate in the ischemic myocardium is improved; the effect for treating ischemic cardiomyopathy is obviously improved.

Description

(1) Technical field [0001] The present invention relates to a pretreatment method for improving the survival ability of elderly bone marrow mesenchymal stem cells after transplantation, and aims to improve the survival rate after transplantation, thereby improving the treatment effect of ischemic cardiomyopathy. (2) Background technology [0002] Ischemic cardiomyopathy is one of the killers that seriously threaten human health, with a high incidence of sudden death. Most patients inevitably develop heart failure despite receiving active treatment. Although traditional treatment methods have greatly improved the survival rate of patients and improved the prognosis, they cannot save the dying myocardium, and have not significantly improved the cardiac remodeling after myocardial infarction. Bone marrow mesenchymal stem cells have attracted wide attention and research in the field of regenerative medicine in recent years because of their self-renewal and multidirectional different...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2501/73C12N5/10C12N15/867
Inventor 刘先宝王建安胡德兴曾志茹
Owner ZHEJIANG UNIV
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