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Hibiscus coccineus tissue culture method

A technology of tissue culture and red okra, which is applied in the field of plant biology, can solve the problems of low biological energy efficiency and not meeting the needs of plant growth, and achieve good results, fast and robust plant growth, energy saving and environmental protection.

Inactive Publication Date: 2016-08-10
ANHUI SCI & TECH UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Plant in vitro rapid propagation mainly uses fluorescent lamps (fluorescent lamps) as artificial light sources, whose spectral distribution contains parts that do not meet the needs of plant growth, and the bioenergy efficiency is extremely low

Method used

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  • Hibiscus coccineus tissue culture method
  • Hibiscus coccineus tissue culture method

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Embodiment approach

[0017] ①Preparation of explants: Select mature and plump red okra seeds, wash them in running water for 1.5-2 hours, then disinfect them with 70% alcohol for 30 seconds in an ultra-clean workbench, and disinfect them with 0.15% mercury chloride for 6-8 minutes. Rinse 5-6 times with sterile water, then soak the sterilized seeds in sterile water for 12-24 hours, insert the sterilized and soaked seeds into 1 / 2MS medium, cultivate them under fluorescent light for 5-7 days, and wait for the cotyledons After flattening, cut out the petioled cotyledons with 1.5-2.0cm hypocotyl as explants, insert them into MS medium supplemented with 0.05 mg / L NAA and culture them for 20 days, and cut out the two leaves grown from the petioled cotyledons The terminal bud of Yixin is the explant;

[0018] ②Light treatment: Then cut the two-leaf and one-center terminal buds grown from cotyledons with stalks and insert them into MS medium supplemented with 0.10 mg / L NAA and 0.05 mg / L 6-BA, and then tran...

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Abstract

The invention discloses a hibiscus coccineus tissue culture method and belongs to the technical field of plant biology. The hibiscus coccineus tissue culture method comprises explants preparation and light quality treatment and concretely comprises selecting mellow and plump hibiscus coccineus seeds, washing the seeds through flowing water for 1.5-2h, carrying out disinfection through alcohol with a concentration of 70% for 30s, carrying out disinfection through mercuric chloride with a concentration of 0.15% for 6-8min, carrying out washing through sterile water 5-6 times, immersing the seeds in sterile water for 12-24h, inoculating a 1 / 2 MS with the seeds, carrying out culture under white light for 5-7 days, cutting off cotyledons with petiole as explants, inoculating a MS with 0.05mg / L of NAA with the explants, carrying out culture for 20 days, cutting off a terminal bud with two true leave as explant, inoculating a MS with 0.10mg / L of NAA and 0.05mg / L of 6-BA with the terminal bud, and culturing the MS under light emitting diode (LED) light with blue, red and green combined light according to 3: 1: 1 and carrying out light culture for 35 days. The LED light with blue, red and green combined light according to 3: 1: 1 is suitable for hibiscus coccineus tissue culture seedling growth. The method has the advantages of simple processes, fast and good plant growth, energy saving and environmental friendliness, has an important effect in hibiscus coccineus rapid propagation in vitro or molecular breeding research and has high actual application value.

Description

1. Technical field [0001] The invention relates to a tissue culture method of red okra, which belongs to the field of plant biotechnology. 2. Background technology [0002] Red okra (Hibiscus coccineus (Medicus) Walt), is a kind of red fruit skin in the okra species, which is an annual herb of the genus Okra in the Malvaceae family. Mainly feed on tender fruits, the flowers and seeds of red okra can be used as medicine, and have good curative effects on various diseases such as gastritis and gastric ulcer. It likes warmth and strong light, heat and drought tolerance, and has wide adaptability to soil. The variety selection of okra is mainly the mode of introduction and breeding and artificial seed production. In recent years, in order to meet the needs of cultivating hybrid seedlings, the use of in vitro rapid propagation technology to preserve okra heterosis resources has become the focus of attention. In rapid plant propagation in vitro, light plays an important role in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 李慧敏周玉丽陈杰
Owner ANHUI SCI & TECH UNIV
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