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Doxycycline hydrochloride pharmaceutical composition and biomedical application thereof

A technology of doxycycline hydrochloride and a composition, applied in the field of biomedicine, can solve problems such as no reports related to the protection of liver by doxycycline hydrochloride, and achieve the effects of protecting the liver, making significant progress, and highlighting substantive characteristics.

Inactive Publication Date: 2016-08-10
ZHENJIANG GAOHAI BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there has been no report on the correlation between doxycycline hydrochloride and its pharmaceutical composition and liver protection

Method used

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  • Doxycycline hydrochloride pharmaceutical composition and biomedical application thereof
  • Doxycycline hydrochloride pharmaceutical composition and biomedical application thereof
  • Doxycycline hydrochloride pharmaceutical composition and biomedical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0021] Separation method: (a) Grind the dried tubers (2kg) of Alisma japonica, extract with 85% ethanol under heat reflux (15L×3 times), combine the extracts, concentrate until there is no alcohol smell (3L), and then use petroleum ether (3L ×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) In step (a), the ethyl acetate extract was removed with D101 type macroporous resin, first eluted with 30% ethanol for 6 column volumes, then eluted with 85% ethanol for 12 column volumes, and collected 85% eluate, Concentrate under reduced pressure to obtain 85% ethanol elution concentrate; (c) 85% ethanol elution concentrate in step (b) is separated with normal phase silica gel, and the volume ratio is 100:1 (12 column volumes), 50: The dichloromethane-methanol grad...

Embodiment 2

[0024] Embodiment 2: pharmacological action

[0025] 1. Materials and methods

[0026] 1.1 Materials and Instruments

[0027] Doxycycline hydrochloride was purchased from China National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. ICR female mice weighing (25 ± 5) g, Shanghai Slack Laboratory Animal Co., Ltd.; Coomassie brilliant blue G-250, triglyceride kit, malondialdehyde test kit, glutathione peroxide kit, Alcohol dehydrogenase assay kit Nanjing Jiancheng Bioengineering Research Institute; Neptune Jinzun, Shenzhen Neptune Pharmaceutical Co., Ltd.; 56° Red Star Erguotou Liquor, Beijing Red Star Brewery. Alpha-D2 lyophilizer, Christ Company, Germany; electronic balance, Shanghai Tianping Instrument Factory; UV752N ultraviolet-visible spectrophotometer, Shanghai Yidian Analytical Instrument Co., Ltd.

[0028] 1.2 Animal grouping

[0029] 72 ICR mice were adaptively fed fo...

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PUM

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Abstract

The invention discloses a doxycycline hydrochloride pharmaceutical composition and a biomedical application thereof. The doxycycline hydrochloride pharmaceutical composition provided by the invention contains doxycycline hydrochloride and a natural product compound (I) which is separated from dry tuber of rhizoma alismatis and novel in structure. When doxycycline hydrochloride and the compound (I) are in effect independently, GSH content in mouse liver tissues can be improved, MDA content can be reduced, accumulation of TG is inhibited, and a certain liver protection effect is realized; and when doxycycline hydrochloride and the compound (I) are in combined effect, the liver protection effect is better, a drug used for protecting the liver can be developed, and substantial characteristics and obvious progress are shown compared with the prior art.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of doxycycline hydrochloride, in particular to a pharmaceutical composition of doxycycline hydrochloride and its application in protecting the liver. Background technique [0002] Doxycycline hydrochloride is a broad-spectrum semi-synthetic tetracyclic antibiotic that has been used in domestic clinics since the 1970s. Its antibacterial spectrum is basically the same as that of tetracycline and oxytetracycline, but its antibacterial effect is 5-10 times stronger than that of tetracycline and oxytetracycline. times, especially effective against tetracycline-resistant Staphylococcus aureus. Doxycycline hydrochloride is well absorbed orally, excreted slowly, and maintains an effective level of blood drug concentration longer than tetracycline and oxytetracycline (half-life 15-20h). It can be taken orally once a day. It is used for upper respiratory tract infection, exudative...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K31/65A61K31/585A61P1/16
CPCC07J71/0005A61K31/585A61K31/65A61K36/884A61K2300/00
Inventor 潘立李义高李小彪赵阳
Owner ZHENJIANG GAOHAI BIOLOGICAL PHARMA CO LTD
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