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Method for determining 5-O-[4'-O-(beta-D-glucopyranose)caffeoyl]quininic acid content in caulis lonicerae

A technology of glucopyranosyl and determination method is applied in the field of content determination of 5-O-[4'-O-caffeoyl]quinic acid in honeysuckle vine, and can solve the problem of target component destruction, poor stability and content determination value. low problems, to achieve the effect of reducing analysis cost, easy operation and good stability

Active Publication Date: 2016-08-10
SHANDONG UNIV +1
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Problems solved by technology

The study found that after the honeysuckle was directly extracted with water ultrasonic, the stability of the component was poor, and the measured value of the content dropped by half after 24 hours, which could not meet the requirements for sample stability (basically stable within 24 hours); after heating and reflux extraction, the stability of the sample solution was relatively low. Good, but the measured value of the content is too low, which is far from reflecting the true content of the ingredient in the medicinal material. It should be that the severe extraction conditions have caused damage to the target ingredient

Method used

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  • Method for determining 5-O-[4'-O-(beta-D-glucopyranose)caffeoyl]quininic acid content in caulis lonicerae
  • Method for determining 5-O-[4'-O-(beta-D-glucopyranose)caffeoyl]quininic acid content in caulis lonicerae
  • Method for determining 5-O-[4'-O-(beta-D-glucopyranose)caffeoyl]quininic acid content in caulis lonicerae

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Embodiment 1

[0036] 1 Instruments and reagents

[0037] Instruments: Sartorius CP225D electronic balance; DGG-9070B electric blast drying oven; Agilent 1200 high performance liquid chromatography.

[0038] Reference substance and source: 5-O-[4′-O-(β-D-glucopyranosyl)caffeoyl]quinic acid is self-made as in the method in the summary of the invention, measured by peak area normalization method, the content is greater than 98%.

[0039] Reagents: acetonitrile and methanol are chromatographically pure, water is purified water prepared by Millipore, and other reagents are analytically pure.

[0040] 2 Chromatographic conditions

[0041] 2.1 Preparation of reference substance solution Accurately weigh 15.36mg of 5-O-[4′-O-(β-D-glucopyranosyl)caffeoyl]quinic acid reference substance, put it in a 100mL measuring bottle, add water to dissolve and dilute To the mark, shake well, as the reference substance stock solution (0.1536mg / mL). Precisely measure 10 mL of the above reference substance stoc...

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Abstract

The invention discloses a method for determining caffeoyl quininic acid glucoside content in caulis lonicerae. The test solution prepared by the method is good in target component stability, the sample recovery rate is 95.0-105.0%, and the determined value can accurately reflect the target component contents in medicinal raw materials. The method includes: crushing caulis lonicerae, and performing pretreatment and extraction; determining the content through an HPLC external standard method or a standard curve method. The method has the advantages that the method is simple to operate and simple in procedure; organic reagents are not used during the preparing processes of the test solution and the control solution, and analyzing cost is lowered.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for determining the content of 5-O-[4'-O-(β-D-glucopyranosyl)caffeoyl]quinic acid in honeysuckle. Background technique [0002] Lonicera japonica Thunb, a Chinese medicinal material, is the dry stem and branch of Lonicera japonica Thunb., harvested in autumn and winter. Sweet in taste, cold in nature, has the effect of clearing away heat and detoxification, dredging wind and dredging collaterals, used for febrile disease fever, toxic heat and bloody dysentery, carbuncle sores, rheumatism-heat arthralgia, joint redness, swelling and heat pain. Its chemical composition mainly includes organic acids, triterpenes, iridoids, flavonoids, volatile oils, etc. [0003] After long-term research, it was found that honeysuckle contains a caffeoylquinic acid glycoside: 5-O-[4'-O-(β-D-glucopyranosyl)caffeoyl]quinic acid, which was tested by HR-ESI- MS, 2D NMR te...

Claims

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Application Information

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IPC IPC(8): G01N30/74G01N30/86G01N30/06
CPCG01N30/06G01N30/74G01N30/8634
Inventor 林永强郭东晓徐丽华汪冰焦阳刘新泳
Owner SHANDONG UNIV
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