A method for improving the fermentation growth efficiency of butyric acid bacteria
A technology of butyric acid bacteria and fermentation tanks, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., to achieve the effects of low concentration, clear ingredients, and increased number of viable cells
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Embodiment 1
[0021] (1) Preparation of medium
[0022] Glucose 20 g / L, peptone 25 g / L, yeast powder 5 g / L, dipotassium hydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 0.5 g / L, light calcium carbonate 1 g / L, manganese sulfate monohydrate 0.02 g / L, pH7.3; anaerobic tube slant medium needs to be added with agar, the agar concentration is 20 g / L; pH 7.3. After deoxygenation, it was sterilized by steam at 0.1 MPa for 20 minutes for later use.
[0023] (2) Seed expansion culture and collection of bottled seed cells
[0024] According to the requirements of aseptic and anaerobic operation, the cells were transferred from the freeze-dried tube of Butyricum strain to the newly prepared slant medium of anaerobic tube. After incubating at 37°C for 12 h, according to the requirements of sterile and anaerobic operation, add 10 mL sterile and anaerobic water to prepare anaerobic tube seed solution.
[0025] 120 mL of anaerobic bottled culture medium 60 mL, in the proportion of 1% (v / v) But...
Embodiment 2
[0030] (1) Preparation of culture medium: Same as Example 1.
[0031] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: Same as in Example 1.
[0032] (3) Fermentation culture: Except for the addition of regulatory factors, the rest of the fermentation conditions are the same as in Example 1.
[0033] Regulatory factor supplementation: Add phenylalanine solution (with a concentration of 2.5 g / L) in a sterile, anaerobic, uniform flow within 1 hour of the start of fermentation, so that the total concentration of phenylalanine is 0.0125 g / L.
[0034] Anaerobic fermentation was carried out for 14 h in the above-mentioned manner, and the number of cells (counted by colony CFU by Hungate rolling tube counting method) was 3.71×10 8 CFU / mL, increased by 71% compared to Example 1; the acetic acid concentration in the fermentation broth was 631 mg / L, which was 16.2% lower than that in Example 1; the butyric acid in the fermentation broth was 2.1 g / L, wh...
Embodiment 3
[0036] (1) Preparation of culture medium: Same as Example 1.
[0037] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: Same as in Example 1.
[0038] (3) Fermentation culture: Except for the addition of regulatory factors, the rest of the fermentation conditions are the same as in Example 1.
[0039]Regulatory factor supplementation: Add phenylalanine solution (concentration: 2.5 g / L) in a sterile, anaerobic uniform flow within 1 hour after the start of fermentation, so that the total concentration of phenylalanine is 0.025 g / L.
[0040] Anaerobic fermentation was carried out for 14 h as above, and the number of cells (counted by colony CFU by Hungate rolling tube counting method) was 3.99×10 8 CFU / mL, increased by 75.5% compared to Example 1; the concentration of acetic acid in the fermentation broth was 443 mg / L, which was 41.2% lower than that in Example 1; butyric acid in the fermentation broth was 1.96 g / L, which was lower than that in E...
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