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Preparation method of Rhodiola rosea extract for effectively promoting sleep and enhancing immunity

A technology for promoting sleep and Rhodiola rosea, which is applied in the field of food biology, can solve the problems of non-industrial application, and achieve the effects of increased content, strong activity, and strong application prospects

Inactive Publication Date: 2016-08-17
HENAN BUSINESS SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The improvement of the effective components of Rhodiola is one of the research directions of many researchers at present, but there is no industrial application at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Carry out ultrafine grinding to Rhodiola rosea, and the crushing particle size is 120 mesh, and obtain Rhodiola rosea powder;

[0017] (2) Take 1Kg of Rhodiola rosea powder, add a certain amount of 6Kg of water, 0.1Kg of glucose, 0.08Kg of peptone, and 0.02Kg of corn steep liquor to prepare a fermentation broth, and then sterilize at 121°C for 20 minutes;

[0018] (3) Inoculate Aspergillus oryzae and Aspergillus niger for 36 hours of aerobic fermentation according to the effective number of viable bacteria in the bacterial solution 3:1, and the weight of the seed solution is 1% of the weight of the fermentation solution; inoculate the fecal chain according to the effective viable number of the bacterial solution 3:2 Cocci and Bacillus coagulans were anaerobically fermented for 24 hours, and the weight of the seed liquid was 2% of the weight of the fermentation liquid. ;

[0019] (4) Add phenylalanine and tyrosine during the fermentation process, so that the concent...

Embodiment 2

[0023] (1) Carry out ultrafine grinding to Rhodiola rosea, and the crushing particle size is 100 mesh, and obtain Rhodiola rosea powder;

[0024] (2) Take 1Kg of Rhodiola rosea powder, add a certain amount of 7Kg of water, 0.13Kg of glucose, 0.09Kg of peptone, and 0.03Kg of corn steep liquor to prepare a fermentation broth, and then sterilize at 121°C for 20 minutes;

[0025] (3) Inoculate Aspergillus oryzae and Aspergillus niger for 36 hours of aerobic fermentation according to the effective viable count of the bacterial liquid at a ratio of 2:1. Cocci and Bacillus coagulans were anaerobically fermented for 24 hours, and the weight of the seed liquid was 2% of the weight of the fermentation liquid. ;

[0026] (4) Add phenylalanine and tyrosine during the fermentation process so that the concentrations of phenylalanine and tyrosine in the fermentation broth are kept at 0.15g / L and 0.2g / L respectively;

[0027] (5) Centrifuging the fermentation broth, taking the supernatant a...

Embodiment 3

[0030] (1) Carry out ultrafine grinding to Rhodiola rosea, and the crushing particle size is 110 mesh, and obtain Rhodiola rosea powder;

[0031] (2) Take 1Kg of rhodiola powder, add a certain amount of 8Kg of water, 0.15Kg of glucose, 0.1Kg of peptone, and 0.05Kg of corn steep liquor to prepare a fermentation broth, and then sterilize at 121°C for 20 minutes;

[0032] (3) Inoculate Aspergillus oryzae and Aspergillus niger for 36 hours of aerobic fermentation according to the effective number of viable bacteria in the bacterial solution 3:2, and the weight of the seed solution is 1% of the weight of the fermentation solution; Cocci and Bacillus coagulans were anaerobically fermented for 24 hours, and the weight of the seed liquid was 2% of the weight of the fermentation liquid. ;

[0033] (4) Add phenylalanine and tyrosine during the fermentation process, so that the concentrations of phenylalanine and tyrosine in the fermentation broth are kept at 0.2g / L and 0.3g / L, respecti...

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Abstract

The invention belongs to the biotechnological field, and concretely discloses a preparation method of Rhodiola rosea extract for effectively promoting sleep and enhancing immunity. The preparation method comprises the following steps: 1, carrying out ultrafine crushing on Rhodiola rosea; 2, adding water, glucose, peptone and corn steep liquor to the above obtained Rhodiola rosea powder to prepare a fermentation solution, and sterilizing the fermentation solution; 3, inoculating Aspergillus oryzae and Aspergillus niger, carrying out aerobic fermentation, inoculating Streptococcus faecalis and Bacillus coagulans, and carrying out anaerobic fermentation; 4, adding phenylalanine and tyrosine in a fed-batch manner in the fermentation process to carry out metabolism regulation; and 5, centrifuging the obtained fermentation solution, and carrying out spray drying to obtain the Rhodiola rosea extract. The content of glycosides and tyrosol in Rhodiola rosea is substantially improved through fermentation of the Rhodiola rosea with various microbes, through metabolism regulation by adding phenylalanine and tyrosine and through microbial fermentation.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a preparation method of a Rhodiola rosea extract which can effectively promote sleep and improve immunity. Background technique [0002] Rhodiola, alias: Rhodiola rosea, Saul Marble (Tibetan name), etc.; it is a perennial herb with a height of 10-20 cm. The root is thick, conical, fleshy, brownish yellow, with many fibrous roots at the root neck, the rhizome is short, thick, cylindrical, covered by many scale-like leaves arranged in tiles. It grows in the alpine and pollution-free zone at an altitude of 1800-2500 meters, and its growth environment is harsh, so it has strong vitality and special adaptability. Rhodiola resources in my country are mostly distributed in alpine regions. Its active ingredients are mainly salidroside and its aglycon-tyrosol, both of which have anti-hypoxia, anti-fatigue, anti-microwave radiation, anti-virus, anti-tumor effects. Rhodiola ...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12P7/22
CPCC12P19/44C12P7/22
Inventor 王永侯银臣冯军伟李向力关炳峰章建军任钊
Owner HENAN BUSINESS SCI RES INST
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