Sterile trace on-line sampling device and sampling method

Abstract

The invention relates to a sterile trace on-line sampling device and a sampling method. The sterile trace on-line sampling device consists of a sterile trace sampling probe, a speed adjustable sampling pump system, a pipeline washing sterilization system, a quantitative sampling ring, a sterile trace sampling probe anti-blocking back blowing system. Trace on-line sampling of a fermentation tank is achieved by using the sampling procedure method. The sterile trace on-line sampling device has the characteristics that sterile sampling is achieved, the dead volume is reduced, trace sampling is achieved, the influence to a fermentation system is very small, continuous sampling is ensured by using the probe anti-blocking back blowing system, and a pipeline is adopted to wash the sterile system so as to prevent contamination and liquid residues. The sampling method is applicable to on-line sampling on trace liquids of a bioreactor on-line detection system for microorganism fermentation, cell culture and the like.

Description

technical field [0001] The invention relates to the field of biochemical analysis, and relates to a sterile trace liquid sampling device and method suitable for on-line detection systems of bioreactors such as microbial fermentation and cell culture. Background technique [0002] The fermentation process is a time-varying, non-linear, uncertain and multi-variable input-output related system, involving the growth, reproduction and metabolism of living organisms, and the mechanism is very complicated. The biochemical variables in the fermentation process are the most critical and direct process parameters that reflect the state of cells. However, due to the limitation of the development level of detection technology, the online measurement of such key parameters has not been well solved. In the actual production process, these variables can only be obtained by offline sampling and manual testing, or estimated by mathematical models through auxiliary variables. Due to the larg...

AI Technical Summary

Problems solved by technology

This type of membrane filtration sampling, because the pore size of the membrane prevents the passage of bacteria, in principle avoids external pollution, but there is still a hidden danger of bacterial contamination in the sampling pipeline; the filter membrane is easily blocked, and there is no effective solution; at the same time, the sampling volume is large and easy Disturb the fermentation system

[0004] The sampling device of the present invention is composed of a sterile micro-sampling probe, an adjustable-speed sampling pump system, a pipeline cleaning and sterilization system, a quantitative sampling loop, and a sterile micro-sampling probe anti-blocking and blowback system. According to the sampling process method, the aseptic on-line Sampling, the sampling volume is small, with sterile backflushing, which effectively solves the shortcomings of the original patent. It is an effective sampling method suitable for microbial fermentation on-line detection systems. So far, no relevant reports have been seen.

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