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A paper chip for chemiluminescence time-resolved and its fabrication method

A time-resolved, chemiluminescence technology, which is applied in chemical instruments and methods, chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problem of inability to change the migration of the solution, the reproducibility and precision of the migration time Not very good and other problems, to achieve the effect of simple method, low cost and good liquidity

Inactive Publication Date: 2018-01-12
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In chip design, the most commonly used method is to design paper chips with different channel lengths to realize chip production, but there are also problems in such a production method.
Since the migration of the reagent in the paper chip does not require an external drive, the migration is achieved entirely by the capillary force of the paper. Therefore, when the chip requires a long channel due to the experiment, the solution will migrate to a certain length and cannot move forward. , no matter how much the amount of reagent is increased, the phenomenon that the solution cannot migrate due to too long length cannot be changed
In addition, when using channels with different lengths for measurement, the reproducibility and precision of the migration time are not very good, and the migration time and precision are the guarantee for the subsequent chemiluminescence measurement

Method used

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  • A paper chip for chemiluminescence time-resolved and its fabrication method
  • A paper chip for chemiluminescence time-resolved and its fabrication method
  • A paper chip for chemiluminescence time-resolved and its fabrication method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Firstly, use CorelDraw X6 drawing software to design a paper chip model. The paper chip model has two detection areas with a diameter of 6 mm and a sample area with a diameter of 6 mm. Each area is connected by a flow channel with a width of 3 mm. The length is 16mm; then use the cutting knife of Ritu CE5000-40-CRP cutting plotter to cut out paper chips on Whatman No. 1 filter paper according to the above paper chip model, then replace the cutting knife with a water-based fiber pen, and put the ink in the pen holder Replace it with a mixture of PDMS and dichloromethane at a volume ratio of 1:30, set the starting point of the drawing, and draw three consecutive passages of PDMS and dichloromethane on a flow channel of the paper chip with a water-based drawing pen along the direction perpendicular to the channel. After the dichloromethane is completely volatilized, a 6mm long delay channel is formed, and the coverage of PDMS on the flow channel is 5×10 -5 g / mm 2 , that i...

Embodiment 2

[0026] Firstly, use CorelDraw X6 drawing software to design a paper chip model. The paper chip model has three detection areas with a diameter of 6mm, and a sample area with a diameter of 6mm. The length is 16mm; then use the cutting knife of Ritu CE5000-40-CRP cutting plotter to cut out paper chips on Whatman No. 1 filter paper according to the above paper chip model, then replace the cutting knife with a water-based fiber pen, and put the ink in the pen holder Replace it with a mixture of PDMS and dichloromethane at a volume ratio of 1:30, set the starting point of the drawing, and use a water-based drawing pen to continuously draw 1 and 2 channels on the two flow channels of the paper chip along the direction perpendicular to the channel. For the mixture of PDMS and dichloromethane, when the dichloromethane is completely volatilized, one flow channel forms a 2mm long delay channel, and one flow channel forms a 4mm long delay channel, and the coverage of PDMS on the flow chan...

Embodiment 3

[0028] Firstly, use CorelDraw X6 drawing software to design a paper chip model. The paper chip model has four detection areas with a diameter of 6 mm, and a sample area with a diameter of 6 mm. Each area is connected by a flow channel with a width of 3 mm. The length is 16mm; then use the cutting knife of Ritu CE5000-40-CRP cutting plotter to cut out paper chips on Whatman No. 1 filter paper according to the above paper chip model, then replace the cutting knife with a water-based fiber pen, and put the ink in the pen holder Replace it with a mixture of PDMS and dichloromethane at a volume ratio of 1:30, set the starting point of the drawing, and draw 1 and 2 lanes on the three flow channels of the paper chip with a water-based drawing pen in a direction perpendicular to the channel respectively. , 3 channels of mixed solution of PDMS and dichloromethane. After the dichloromethane is completely volatilized, 2mm, 4mm, and 6mm long delay channels are formed in turn. The coverage ...

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Abstract

The invention discloses a paper chip used for chemiluminescence time resolution and a manufacturing method thereof. Each circulation channel except one of the paper chip is covered with polydimethylsiloxane having different lengthes, and the manufacturing method is simple, green and environmentally friendly, and has low cost. The cutting of the paper chip and accurate carving of polydimethylsiloxane can be realized with the only needing of a simple carving machine. The accurate control of the migration rate of the sample in the paper chip is realized by means of controlling the cover capacity of the polydimethylsiloxane by the paper chip, so that the time resolution of the migration rate of the sample can be realized on the premise of the same length of the channel, the chemiluminescence detection of simultaneous determination of different substances is realized, and the used polydimethylsiloxane does not affect the chemiluminescence signal of the reaction. Moreover, the reproducibility of the migration time is good, and the precision is high.

Description

technical field [0001] The invention belongs to the technical field of making chemiluminescent paper chips, and in particular relates to a method of adding a delayed reagent whose position and amount can be precisely controlled on a paper chip. A simple cutting machine is used to realize the production of the above-mentioned paper chip, so that it can be To achieve the purpose of time-resolved detection of chemiluminescence on paper. Background technique [0002] In 2007, the Whitesides research group of Harvard University first proposed the concept of "paper-based microfluidic device" (or "paper chip laboratory"). As an emerging research field, paper chip laboratory has gained rapid development in recent years. The development of it provides a novel research method for medical diagnosis, environmental monitoring and food safety monitoring. The paper-on-a-chip laboratory uses paper as a reaction carrier, and constructs corresponding hydrophilic and hydrophobic regions on th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/76
CPCB01L3/5027B01L3/502707B01L2300/0861B01L2300/168B01L2400/0472G01N21/76
Inventor 刘伟褚伟茹赵鹛陈莹
Owner SHAANXI NORMAL UNIV
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