Specificity detection primers of Aids related intestinal tract Megamonas funiformis translocation and application

Abstract

The invention discloses specificity detection primers of Aids related intestinal tract Megamonas funiformis translocation and application. The sequence of an upstream primer is Meg-F5'-AGTTCCTTCTCTTCGGAGAAC-3', and the sequence of a downstream primer is Meg-R5'-TAAGGAGGTGATCCAGCC-3'; the specificity detection primers have the advantages that the detection is accurate, simple, convenient and rapid, the sensitivity is high, and the specificity is high and have excellent detectability and a wide application prospect; the technology can be widely applied to early stage monitoring before symptom appearance of Aids patient intestinal tract Megamonas funiformis flora translocation, and a scientific basis is provided for formulating the treatment strategy.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a specific detection primer set and application for detecting the translocation of the intestinal megamonas fusiformis of AIDS patients. Background technique [0002] AIDS, or Acquired Immune Deficiency Syndrome, has been on the rise internationally. As far as the country is concerned, as of the end of October 2015, a total of 575,000 HIV-infected people and patients have been reported nationwide. , 177,000 people died. In recent years, more and more experts and scholars have shifted the focus of AIDS research to the intestinal tract. It is generally believed that the intestinal tract is the target organ for HIV to attack and replicate, and the intestinal flora disorder and intestinal barrier damage caused by the same time make the intestinal tract The translocation of flora induces a series of complications such as infection and chronic immune system activation, thus accelerati...

AI Technical Summary

Problems solved by technology

At present, the diagnosis of intestinal flora translocation is mainly based on the basic disease and clinical manifestations of the patient plus laboratory diagnosis. The commonly used method is the detection of endotoxin in the blood of the patient. Although this method is fast, it cannot be specific. The type of bacteria infecting is often diagnosed after the onset of the disease, which is not conducive to early diagnosis and treatment; in addition, blood culture detection methods are often used clinically. Factors affecting the results are not necessarily reliable, which not only increases the cost of diagnosis, but also delays the timing of diagnosis

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