Method for producing amino acid water-soluble fertilizer by utilizing Drawida Japonica Michelsen

A technology of amino acids and water-soluble fertilizers, applied in nitrogen fertilizers, liquid fertilizers, fertilizer mixtures, etc., can solve the problems of unutilized earthworm crude protein and waste of resources, and achieve the effects of improving the quality of fruiting bodies, improving soil, and increasing the utilization rate of drugs

Inactive Publication Date: 2016-09-28
陈可夫
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  • Claims
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Problems solved by technology

[0003] The purpose of the present invention is to aim at the fact that a large amount of earthworm crude protein cannot be utilized after extracting vermixin by Japanese Duraphyllum worms, which leads to waste of resources.

Method used

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  • Method for producing amino acid water-soluble fertilizer by utilizing Drawida Japonica Michelsen
  • Method for producing amino acid water-soluble fertilizer by utilizing Drawida Japonica Michelsen
  • Method for producing amino acid water-soluble fertilizer by utilizing Drawida Japonica Michelsen

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[0017] 1. Earthworm rinsing: Rinse with surfing method for 1 hour, rinse the outer skin of fresh earthworms, and the food in the gastrointestinal tract will be excreted transparently after soaking in water;

[0018] 2. Homogenization: Add the rinsed earthworms into the pH8.14 Tris-HCl buffer solution, and homogenize with a homogenizer;

[0019] 3. Extraction of vermisin (Fe-Zn glycoprotein): see the invention patent (patent number 97111350.5) for the extraction method.

[0020] 4. Hydrolysis of compound amino acids: Concentrate the remaining earthworm crude protein solution after extraction into a colloidal solution, raise the temperature to 50°C, adjust the pH to 8.3, and add alkaline protease solution for 6 hours according to the enzyme power of 10000U / g rear,. Heat to 95°C to kill alkaline enzyme activity, cool to 46°C, adjust pH to 3.4, add acid protease solution for enzymatic hydrolysis at 1000U / g enzyme strength for 5 hours, heat to 95°C to kill acid enzyme activit...

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Abstract

The invention relates to a method for producing an amino acid water-soluble fertilizer by utilizing Drawida Japonica Michelsen. The method comprises the following steps: A. hydrolyzing compound amino acid, namely, adding alkaline protease liquid into crude earthworm protein liquid left after lumbricin is extracted for carrying out enzymolysis, adding acid protease, and further hydrolyzing to prepare hydrolyzed compound amino acid; B. carrying out a chelation reaction, namely, adding ZnSO4.7H2O and Na2SeO3 into the hydrolyzed compound amino acid for carrying out the chelation reaction to obtain compound amino acid chelate; C. separating and purifying, namely, separating and purifying the compound amino acid chelate by using an organic solvent methanol or ethanol, and then drying to obtain the amino acid water-soluble fertilizer. The method has the advantages that the water-soluble fertilizer prepared by carrying out chelation on the compound amino acid and trace elements can be rapidly absorbed by plants, and has the characteristics of increasing yield, improving sporocarp quality, being stress-resistant, improving soil, increasing medicine utilization rate and the like.

Description

technical field [0001] The invention relates to the field of water-soluble fertilizers. Background technique [0002] At present, after using the Japanese Dura worm to extract vermixin, there is still a large amount of earthworm crude protein that has not been utilized, which is a waste of resources. Contents of the invention [0003] The purpose of the present invention is to aim at the fact that a large amount of earthworm crude protein cannot be utilized after extracting vermixin by Japanese Duraphyllum worms, which leads to waste of resources. . [0004] The present invention comprises the steps: [0005] A. Hydrolysis of compound amino acids: extract vermisin from Duraphyllum japonica, heat up the remaining earthworm crude protein solution after extracting vermisin to 48-52°C, adjust pH to 8.12-8.14, and adjust the enzyme strength to 9500- Add 11000U / g alkaline protease solution for enzymolysis for 4.5-6 hours, heat to 90-100°C to inactivate the enzyme activity, co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05C11/00C12P13/04
CPCC05C11/00C05G5/20C12P13/04
Inventor 陈可夫
Owner 陈可夫
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