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Method for simultaneously detecting fosaprepitant and aprepitant in plasma

A technology of aprepitant and plasma, which is applied in the field of drug analysis, can solve difficult problems and achieve the effect of high sensitivity and easy operation

Inactive Publication Date: 2016-09-28
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for patients with severe vomiting symptoms, continuous administration of aprepitant preparations is difficult to achieve

Method used

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  • Method for simultaneously detecting fosaprepitant and aprepitant in plasma
  • Method for simultaneously detecting fosaprepitant and aprepitant in plasma
  • Method for simultaneously detecting fosaprepitant and aprepitant in plasma

Examples

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Effect test

Embodiment 1

[0029] Example 1: Simultaneous determination of fosaprepitant and aprepitant concentrations in human plasma.

[0030] 1. Experimental materials and instruments

[0031] Material: Fosaprepitant Diglumine Reference substance: Jiangsu Hansoh Pharmaceutical Co., Ltd., batch number: RS20140413, content: 99.8%. Aprepitant reference substance: Jiangsu Hansoh Pharmaceutical Co., Ltd., batch number: RS20140830, content: 99.8%. Glibenclamide (internal standard) reference substance: National Institute for the Control of Pharmaceutical and Biological Products, batch number: 100135-200404, for content determination. Acetonitrile: Merck Company, HPLC grade. Methanol: Merck Company, HPLC grade. Ammonia water: Shanghai Jiuyi Chemical Reagent Co., Ltd., analytically pure. Ammonium acetate: ROE Scientific Inc., HPLC grade. EDTA: Tokyo Chemical Industry Co., Ltd. Ammonium dihydrogen phosphate: Chengdu Kelong Chemical Reagent Factory, analytically pure. Water was prepared by a MilliporeMil...

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Abstract

The invention discloses a method for simultaneously detecting fosaprepitant and aprepitant in plasma. The method comprises detecting a pretreated correction standard sample and a pretreated plasma sample through high performance liquid chromatography-tandem mass spectrometry, carrying out quantification through an internal standard method, building a standard curve equation through fosaprepitant and aprepitant correction standard sample concentrations as X coordinates and fosaprepitant and aprepitant peak area-to-internal standard peak area ratios as Y coordinates, introducing the plasma sample fosaprepitant and aprepitant peak area-to-internal standard peak area ratios into the standard curve equation and calculating fosaprepitant and aprepitant concentrations. The method is fast and accurate, has high sensitivity and strong specificity, is convenient for operation and is suitable for simultaneous determination of fosaprepitant and aprepitant concentrations of blood plasma.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a method for analyzing and measuring drugs in vivo, in particular to a method for simultaneously detecting fosaprepitant and aprepitant in blood plasma. Background technique [0002] Aprepitant oral preparation is the first substance P / NK-1 receptor antagonist approved by the US FDA. Clinically, aprepitant combined with other antiemetic drugs can prevent acute and delayed nausea and vomiting caused by anticancer drugs and chemotherapy drugs. However, for patients with severe vomiting symptoms, continuous administration of aprepitant preparations is difficult to achieve. Fosaprepitant dimeglumine is a water-soluble prodrug of aprepitant, which can be rapidly converted into aprepitant in the body after intravenous infusion. Therefore, simultaneous determination of the concentration of fosaprepitant and aprepitant in the body and evaluation of the pharmacokinetic process of th...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 许美娟储继红吴婷居文政李长印张军刘史佳戴国梁
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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