Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A dyeing solution and eosin technology, applied in the field of eosin staining solution, can solve problems such as environmental pollution, physical injury to workers, poisonous chemical reagents, etc., and achieve the effects of increasing water solubility, preventing slice deterioration, and good antibacterial properties
Active Publication Date: 2018-10-09
南京汉瑞生物科技有限公司
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, not only causing harm to the health of the staff, but also polluting the environment
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
[0020] A method for preparing the eosin staining solution described in Example 1: adding sodium chloride to water at a temperature of 40° C., stirring evenly, heating up to 50° C., adding water-soluble eosin and citric acid aqueous solution, stirring evenly, Add nano-silver while stirring, and in a water bath with a temperature of 30°C, ultrasonicate the water bath for 10 minutes to obtain the first mixed solution;
[0021] Add boric acid solution into absolute ethanol, stir evenly, add sodium dodecylbenzenesulfonate and fatty acid glycerides, in a water bath at a temperature of 30°C, ultrasonicate the water bath for 2 minutes, add glycerin, stearic acid, spermaceti Alcohol, isopropanolamine, Tween 20, allantoin, ethylparaben, acrylic resin and tocopheryl acetate, at a water bath temperature of 40°C, ultrasonication in a water bath for 1 min to obtain a second mixed solution;
[0022] The second mixture was added to the first mixture while stirring, and the temperature of the ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses an eosin staining solution. The eosin staining solution is prepared from the following components in parts by mass: 12 parts of water-soluble eosin, 1200 parts of water, 0.4 part of sodium chloride, 1.3 parts of a 1mol / L citric acid water solution, 20 parts of absolute ethyl alcohol, 0.01 part of nano silver, 19 parts of glycerol, 2 parts of a boric acid solution with the concentration of 5mmol / L, 2 parts of stearic acid, 0.3 part of cetyl alcohol, 1 part of isopropanolamine, 2 parts of Tween 20, 0.8 part of allantoin, 1 part of ethylparaben, 6 parts of acrylic resin, 0.8 part of tocopheryl acetate, 0.001 part of sodium dodecylbenzene sulfonate and 0.001 part of fatty glyceride. The eosin staining solution meets the requirement of environment protection of pathological reagents and the staining effect is better than that of a traditional eosin staining solution.
Description
technical field [0001] The invention relates to an eosin staining solution. Background technique [0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, which not only cause harm to the health of the staff, but also pollute the environment. Contents of the invention [0003] It is an object of the present invention to solve at least the above-mentioned problems and to provide at least the advantages which will be described later. [0004] Another object of the present invention is to provide an eosin staining solution, which not only meets the requirements of environmental protection of pathological reagents, but also has better staining effect than traditional eosin staining solution. [0005] In order to achie...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more