Kit for measuring serum amyloid A and preparation method of kit

A technology for amyloid and serum amyloid, applied in the fields of medicine and biochemistry, can solve the problems of low measurement accuracy and complicated operation, and achieve the effects of high detection accuracy, rapid response, and convenient operation

Inactive Publication Date: 2016-10-12
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a kit for measuring serum amyloid A and its preparation method in order to overcome the defects of complex operation and low measurement accuracy in the prior art

Method used

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  • Kit for measuring serum amyloid A and preparation method of kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0066] Reagent R1:

[0067] Tris buffer 100 mmol / L

[0068] Potassium chloride 180 mmol / L

[0069] Polyethylene glycol-2000 20 g / L

[0070] Sodium azide 0.7 g / L

[0071] Bovine Serum Albumin 30 g / L

[0072] The solvent is purified water

[0073] Reagent R2:

[0074] Tris buffer 125 mmol / L

[0075] Bovine Serum Albumin 11 g / L

[0076] Sodium azide 0.7g / L

[0077] Latex coated anti-serum amyloid A antibody 4g / L

[0078] Its solvent is purified water.

Embodiment 2

[0080] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0081] Reagent R1:

[0082] PBS buffer 185 mmol / L

[0083] Sodium chloride 350 mmol / L

[0084] Polyethylene glycol-8000 10 g / L

[0085] Sodium azide 0.9 g / L

[0086] Bovine Serum Albumin 56 g / L

[0087] The solvent is purified water

[0088] Reagent R2:

[0089] PBS buffer 15 mmol / L

[0090] Bovine serum albumin 4 g / L

[0091] Sodium azide 0.6 g / L

[0092] Latex-coated anti-serum amyloid A antibody 3 g / L

[0093] Its solvent is purified water.

Embodiment 3

[0095] Kit preparation and method of use

[0096] 1. Preparation of latex-coated anti-serum amyloid A antibody: first dilute polystyrene microspheres with a particle size of 120 nm with 50 mmol / L MES buffer to a mass concentration of polystyrene microspheres of 5% solution, and then add 0.5 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride to each milliliter of the solution, and react for 1 hour at 25°C, using Centrifuge at 20,000 rpm / min for 30 minutes, discard the supernatant, dilute the pellet with 50 mmol / L MES buffer, then use a centrifuge, centrifuge at 20,000 rpm / min for 30 minutes, discard For the supernatant, continue to dilute the pellet with 50mmol / L MES buffer, so that the mass concentration of polystyrene microspheres is 2.0%, and then use a centrifuge to centrifuge at 20,000 rpm / min for 30 minutes, discard the supernatant , retain the precipitate, then dilute the precipitate with 50mmol / L MES buffer, and add an equal volume of MES buffer contain...

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Abstract

The invention discloses a kit for measuring serum amyloid A and a preparation method of the kit. The kit comprises two independent liquid components, namely a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following ingredients by content: a buffer, inorganic salt ions, a coagulant, a preservative and a stabilizer; the reagent R2 comprises the following ingredients: the buffer, the stabilizer, the preservative, and a latex-coated anti-serum amyloid A antibody. The preparation method comprises the following steps: preparing the reagents according to the contents of the ingredients; mixing a to-be-tested sample with the reagent R1 and the reagent R2; measuring absorbance variation with a fully automatic biochemical analyser; calculating the concentration of serum amyloid A in the sample according to the absorbance variation. The kit and the preparation method have the advantages of high measurement accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring serum amyloid A and a preparation method thereof. Background technique [0002] Serum amyloid A (serum amyloid A, SAA) is an acute phase reaction protein, which belongs to the heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12000. In the acute phase reaction, it is stimulated by IL-1, Stimulated by IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, which can increase to 100-1000 times the initial concentration, but the half-life is very short, only about 50 minutes, serum amyloid A is associated with high-density lipoprotein (HDL), which regulates HDL metabolism during inflammation, and a particularly important property of serum amyloid A is the ability of its degradation products to form amyloid A (AA) fibrils. It is deposited in different organs, which is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/68
CPCG01N21/31G01N33/68
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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