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A method for identifying the plant source of medicinal material Xihuangcao

A technology of Xihuang grass and plants, applied in the fields of pharmacognosy and analytical chemistry, can solve problems such as difficulty in identification, variation of chromatographic patterns, and inability to match

Active Publication Date: 2017-09-26
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] 1) The time to analyze a sample is too long, it takes 100-120 minutes
[0014] 2) The choice of reference substance needs to be discussed
Due to the complex composition and variety of Xihuang herb medicinal materials, and the long analysis time, any slight change in chromatographic conditions will mean that the overall chromatographic pattern varies and cannot match the control fingerprint, resulting in misjudgment and difficulties in identification. , causing the reproducibility of the experiment to be affected

Method used

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  • A method for identifying the plant source of medicinal material Xihuangcao
  • A method for identifying the plant source of medicinal material Xihuangcao
  • A method for identifying the plant source of medicinal material Xihuangcao

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1 The Establishment of High Performance Liquid Chromatogram of Reference Substance

[0082] 1. Chromatographic conditions:

[0083] Column: AgiLent EcLipse XDB-C 18 (4.6mm×250mm, 5μm) chromatographic column.

[0084] Mobile phase: use acetonitrile as phase A and 0.08-0.3% by volume phosphoric acid aqueous solution as phase B for gradient elution. After testing, it was found that when phase B was 0.1% (vol%) phosphoric acid aqueous solution, the absorption peaks of each reference substance had good peak shape, high resolution and stable baseline, so phase B is preferably 0.1% (vol%) phosphoric acid aqueous solution. The mobile phase gradient elution conditions are shown in Table 1.

[0085] Table 1 Gradient elution program

[0086]

[0087] Flow rate: 0.6 ~ 1.2mL / min; after testing, it is found that the separation effect is the best when the flow rate is 0.8mL / min, so the preferred flow rate is 0.8mL / min.

[0088] Column temperature: 23-28°C, preferabl...

Embodiment 2

[0101] Example 2 Establishment of High Performance Liquid Chromatogram of Phytophthora chinensis

[0102] 1. Chromatographic conditions

[0103] With the preferred chromatographic conditions of Example 1, wherein the ultraviolet detection wavelength is 240nm and 330nm.

[0104] 2. Preparation of mixed reference solution

[0105] With embodiment 1 " preparation of mixed reference substance solution ".

[0106] 3. Preparation of the test solution

[0107] Weigh 0.5g of the dried Phytophthora chinensis powder, accurately weigh it, place it in a corked Erlenmeyer flask, add 20mL of 50% ethanol, seal it tightly, weigh it, extract it by ultrasonic for 30min, let it cool, weigh it again, and use 50% Make up the lost weight with ethanol, filter through a 0.22 μm filter membrane, and take the subsequent filtrate to obtain the product.

[0108] 4. Determination

[0109] Precisely draw 10 μl of the mixed reference solution prepared in step 2 and the test solution prepared in step...

Embodiment 3

[0111] Example 3 Establishment of high-performance liquid chromatograms of thread-lined fragrant tea

[0112] 1. Chromatographic conditions

[0113] With the preferred chromatographic conditions of Example 1, wherein the ultraviolet detection wavelength is 240nm and 330nm.

[0114] 2. Preparation of mixed reference solution

[0115] With embodiment 1 " preparation of mixed reference substance solution ".

[0116] 3. Preparation of the test solution

[0117] Weigh 0.5g of the dried thread-line fragrant tea powder, weigh it accurately, place it in a conical flask with a stopper, add 20mL of 50% ethanol, seal it tightly, weigh it, extract it by ultrasonic for 30min, let it cool down, weigh it again, and use 50 % ethanol to supplement the lost weight, filter through a 0.22 μm filter membrane, and take the subsequent filtrate to obtain the product.

[0118] 4. Determination

[0119] Precisely draw 10 μl of the mixed reference solution prepared in step 2 and the test solutio...

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PUM

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Abstract

The invention provides a method for identifying a plant source of herbal rabdosia lophanthide. The method is based on the high performance liquid chromatography. The method comprises the following steps: using rosmarinic acid, rabdosia lophanthoides apiin A, oridonin, pedalitin and caffeoyl acetate as reference substances; comparing chromatograms of a solution to be tested and a solution mixed with the reference substances, detecting an absorption peak of rosmarinic acid, oridonin and pedalitin under any detection wavelength of 235nm to 240nm and the absorption peak of rosmarinic acid and pedalitin under the detection wavelength of 330nm, and determining the substance to be tested is the plant rabdosia lophanthide; and detecting the absorption peak of rosmarinic acid, rabdosia lophanthoides apiin A and caffeoyl acetate under any detection wavelength of 235nm to 240nm, detecting the absorption peak of rosmarinic acid and caffeoyl acetate under the detection wavelength of 330nm, determining the substance to be tested to be the plant rabdosia lophanthoides or the rabdosia lophanthoides. The method has the advantages of simplicity and convenience in operation, good characteristics, good reproducibility, short time and the like.

Description

technical field [0001] The invention belongs to the fields of pharmacognosy and analytical chemistry, and in particular relates to a method for identifying the plant source of medicinal material Xihuangcao based on high-performance liquid chromatography. Background technique [0002] The medicinal material Xihuangcao is a commonly used herbal medicine in the folk. It is named for the yellow sap from fresh leaves rubbed in the wet place beside the stream in the valley. It is slightly bitter in taste and cool in nature. It has the effects of clearing heat, removing dampness, promoting gallbladder and reducing jaundice. It is used to treat diseases such as damp-heat diarrhea, bruises, bruises, acute jaundice hepatitis and acute cholecystitis. Xiaoyan Lidan Tablets produced with Xihuangcao as the main raw material has become the best-selling Chinese patent medicine in hepatobiliary medicine. [0003] In order to ensure the quality of medicines, the prior art has established som...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 张慧晔唐海明王德勤李楚源
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE