Disinfection method for castanopsis hystrix explants

Abstract

The invention discloses a disinfection method and tissue culture reproduction method for castanopsis hystrix explants. When the explants are seeds, the disinfection method comprises the steps that the seeds are soaked with boiling water, seed coats are removed, and then a mercuric chloride solution is utilized for soak disinfection; when the explants are stems, the disinfection method comprises the step that soaking with alcohol and soaking with the mercuric chloride solution are conducted in sequence for mercuric chloride solution. The tissue culture reproduction method comprises the steps that the castanopsis hystrix plants which grow robustly and are free of pest and disease damage and high in seed setting rate are selected, and seeds or stems are collected, disinfected by means of the above-mentioned disinfection methods and then inoculated onto an improved MS culture medium for tissue culture. According to the disinfection method and tissue culture reproduction method for the castanopsis hystrix explants, a castanopsis hystrix tissue culture explant disinfection system is established, the premise and guarantee are provided for further conducting series of research on castanopsis hystrix explant induction, propagation, rooting and the like, and the important and practical research and production significance in achieving large-scale seedling of optimum seeds and industrialization development is achieved.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows scientists to study different types of vegetable roots that grow naturally or help them reproduce themselves during their life cycle. It also helps prevent diseases caused by bacteria like those causing yellow stains (yellow curd). By culturing these germplasm samples with specific conditions, they may be used to create new plantings called “red coner” tubers. These young crops will produce high yields compared to older ones due to its superior properties.

Problems solved by technology

This patented describes different methods that may help creature trees grow better when they come from rainfored or green woodland regions where plants tend to die over during winter periods without being affected even if some parts get wet again after harvesting them outdoors. These techniques include root pruning, cuttings, cutpropelling, grafting, infusion, dyeing, pollinating, cultivars like yellowconks (Cerus), white consisting mostly pigment called rhodocyanine, and purple copper(I) plumquirit).

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