Disinfection method for castanopsis hystrix explants

A disinfection method and explant technology, applied in the field of plant tissue culture, can solve the problems of endogenous bacteria pollution, pollution problem loss, incomplete disinfection of explants, etc. Effect

Inactive Publication Date: 2016-11-09
GUANGDONG ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows scientists to study different types of vegetable roots that grow naturally or help them reproduce themselves during their life cycle. It also helps prevent diseases caused by bacteria like those causing yellow stains (yellow curd). By culturing these germplasm samples with specific conditions, they may be used to create new plantings called “red coner” tubers. These young crops will produce high yields compared to older ones due to its superior properties.

Problems solved by technology

This patented describes different methods that may help creature trees grow better when they come from rainfored or green woodland regions where plants tend to die over during winter periods without being affected even if some parts get wet again after harvesting them outdoors. These techniques include root pruning, cuttings, cutpropelling, grafting, infusion, dyeing, pollinating, cultivars like yellowconks (Cerus), white consisting mostly pigment called rhodocyanine, and purple copper(I) plumquirit).

Method used

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  • Disinfection method for castanopsis hystrix explants
  • Disinfection method for castanopsis hystrix explants
  • Disinfection method for castanopsis hystrix explants

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1 seed disinfection method

[0042] 1. Do not remove the outer testa

[0043] After the pre-treatment, the following six methods were used for advanced treatment on the ultra-clean workbench (as shown in Table 1). Each bottle was inoculated with 1 seed, 1 bottle was 1 repetition, and each treatment was 20 bottles ( figure 1 ).

[0044] Table 1 Different seed disinfection methods

[0045]

[0046]

[0047] The results showed that the contamination rate of K1-K3 seeds was as high as 100%, and the disinfection effect was poor, and the contamination rate of K4-K6 was 20%-95%, which was better than that of K1-K3. The test results show that soaking seeds in hot water at 100°C can effectively kill the bacteria on the surface of the seeds and reduce the contamination rate of the seeds.

[0048] The pollution rate of K4 is 90%, and the disinfection effect is not ideal. The pollution rate of K6 seeds is the lowest, only 20%, and the disinfection effect is the b...

Embodiment 2

[0057] The disinfection method of the explant of the stem section of the red cone tree of embodiment 2

[0058] 1. Remove the leaves of the collected branches indoors, soak them in washing powder or disinfectant powder for 20 minutes, rinse them with tap water for 20 minutes, and place them on an ultra-clean workbench after cleaning. Under sterile conditions, cut into 1-2cm long stem segments, each stem segment contains 1 bud point. After being treated with 75% ethanol for 30 seconds, rinse with sterile water 2 to 3 times. Eighteen kinds of disinfection treatment methods were set up for stem section disinfection treatment (Table 3). The sterilized stem section of Rhododendron angustifolia was inserted into the improved MS medium on the ultra-clean workbench, and the oblique insertion method was adopted for inoculation. One stem segment was inoculated per bottle, and 30 were inoculated for each treatment, including 10 lateral bud branches, semi-lignified branches, and 10 south...

Embodiment 3

[0063] Embodiment 3 red cone tissue culture propagation method

[0064] When the method of this paper carries out the tissue culture propagation of the red cone, the explant can choose the seed or the stem segment.

[0065] 1, described red cone tissue culture propagation method comprises the selection of red cone explant, collection, pretreatment, aseptic treatment, specifically comprises the following steps:

[0066] (1) Selection of explants

[0067] According to different research objectives, different explants of seed embryos or stem segments are selected, which generally come from excellent plants with robust growth, no pests and diseases, and high seed setting rate.

[0068] (2) Explant collection

[0069] Seed collection: Collect when the seeds are fully mature from the beginning of December to the end of December, when the fruit involucre and thorns turn from green to brown.

[0070] Collecting stems:

[0071] ①Explant larvae: During the peak growth season of the ...

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Abstract

The invention discloses a disinfection method and tissue culture reproduction method for castanopsis hystrix explants. When the explants are seeds, the disinfection method comprises the steps that the seeds are soaked with boiling water, seed coats are removed, and then a mercuric chloride solution is utilized for soak disinfection; when the explants are stems, the disinfection method comprises the step that soaking with alcohol and soaking with the mercuric chloride solution are conducted in sequence for mercuric chloride solution. The tissue culture reproduction method comprises the steps that the castanopsis hystrix plants which grow robustly and are free of pest and disease damage and high in seed setting rate are selected, and seeds or stems are collected, disinfected by means of the above-mentioned disinfection methods and then inoculated onto an improved MS culture medium for tissue culture. According to the disinfection method and tissue culture reproduction method for the castanopsis hystrix explants, a castanopsis hystrix tissue culture explant disinfection system is established, the premise and guarantee are provided for further conducting series of research on castanopsis hystrix explant induction, propagation, rooting and the like, and the important and practical research and production significance in achieving large-scale seedling of optimum seeds and industrialization development is achieved.

Description

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Claims

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Application Information

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Owner GUANGDONG ACAD OF FORESTRY
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