Arborescent polypeptide for conducting cell therapy on HPV infection and screening method, preparation method and application of arborescent polypeptide

A dendritic polypeptide and cell therapy technology, which is applied in the direction of peptide preparation methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of large tissue cell damage, unsatisfactory curative effect, and high medical expenses, and achieve the goal of eliminating Effects of viruses, single structure and properties, and small molecular weight

Inactive Publication Date: 2016-11-09
江苏安泰生物技术有限公司
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Problems solved by technology

The common defect of these therapies is the lack of specificity, large damage to normal tissue cells, severe side effects, not only unsatisfactory curative effect, but also huge medical expenses.

Method used

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  • Arborescent polypeptide for conducting cell therapy on HPV infection and screening method, preparation method and application of arborescent polypeptide
  • Arborescent polypeptide for conducting cell therapy on HPV infection and screening method, preparation method and application of arborescent polypeptide
  • Arborescent polypeptide for conducting cell therapy on HPV infection and screening method, preparation method and application of arborescent polypeptide

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Embodiment Construction

[0020] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0021] 1. Design and screening of epitope peptides

[0022] 1. Human papillomavirus antigen and its homology analysis

[0023] HPV16 and 18 are the most common high-risk types and are the main pathogenic factors of cervical cancer and its precancerous lesions. In particular, the detection rate of HPV16 in cervical cancer tissues exceeds 50%, which plays an important role in the occurrence of cervical cancer. Studies have shown that HPV16 E7 protein is the main oncoprotein for tumor cell transformation, and it plays a transformation role by binding and inactivating the tumor suppressor protein Rb.

[0024] Select the HPV16 E7 protein from Genebank, its 98 amino acid sequence: MHGDT PTLHE YMLDT QPETTDLYCY EQLND SSEEE DEIDG PAGQA EPDRA HYNIV TFCCK CDSTL RPLCVQ S...

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Abstract

The invention relates to an arborescent polypeptide for conducting a cell therapy on HPV infection. The arborescent polypeptide is characterized by being formed by connecting four carboxyl groups of a C-terminal of an HPV epitope peptide with three amino groups of a poly-L-arginine framework composed of arginine residues, wherein an amino acid sequence of the epitope peptide is Tyr-Met-Leu-Asp-Leu-Gln-Pro-Glu-Thr-Gly-Gly-Gly. The invention further provides a screening method, a preparation method and application of the arborescent polypeptide. Through design and analysis of multiple kinds of combined bioinformatic software, the capacity of finding a new epitope is improved, the epitope loading orientations achieve consistency, and the specific combination capacity and reaction sensitivity of the epitope peptide are exponentially improved in unit space. The epitope peptide is single in structure and property and has the great advantages of being high in specificity, free of potential biological hazards and capable of achieving stardardization in preparation process.

Description

technical field [0001] The invention relates to the technical field of prevention and treatment of cervical cancer, and relates to a dendritic polypeptide for cell therapy of HPV infection, a screening method, a preparation method and application thereof. Background technique [0002] Cervical cancer is the most common malignant tumor of reproductive tract in women, which seriously threatens women's health and life. my country is a country with a high incidence of cervical cancer, especially in recent years, there is a clear upward trend in young cervical cancer patients, and the incidence rate is increasing at a rate of 2%-3% per year. Human papillomavirus (human papillomavirus, HPV) can cause benign and malignant hyperplasia, especially high-risk HPV is closely related to cervical cancer. At present, the treatment of cervical cancer mostly adopts the combined application of surgery, chemotherapy, radiotherapy, biological therapy and traditional Chinese medicine. The comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/00A61K39/12A61P31/20A61P35/00
CPCA61K39/12C07K7/08C07K19/00C12N2710/20034
Inventor 罗进闫小君
Owner 江苏安泰生物技术有限公司
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