Arborescent polypeptide for conducting cell therapy on HPV infection and screening method, preparation method and application of arborescent polypeptide
A dendritic polypeptide and cell therapy technology, which is applied in the direction of peptide preparation methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of large tissue cell damage, unsatisfactory curative effect, and high medical expenses, and achieve the goal of eliminating Effects of viruses, single structure and properties, and small molecular weight
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[0020] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.
[0021] 1. Design and screening of epitope peptides
[0022] 1. Human papillomavirus antigen and its homology analysis
[0023] HPV16 and 18 are the most common high-risk types and are the main pathogenic factors of cervical cancer and its precancerous lesions. In particular, the detection rate of HPV16 in cervical cancer tissues exceeds 50%, which plays an important role in the occurrence of cervical cancer. Studies have shown that HPV16 E7 protein is the main oncoprotein for tumor cell transformation, and it plays a transformation role by binding and inactivating the tumor suppressor protein Rb.
[0024] Select the HPV16 E7 protein from Genebank, its 98 amino acid sequence: MHGDT PTLHE YMLDT QPETTDLYCY EQLND SSEEE DEIDG PAGQA EPDRA HYNIV TFCCK CDSTL RPLCVQ S...
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